Cysteine enhance Holstein bull’s sperm assessment quality and conception rate when added to duck egg yolk low-density lipoproteins

Hussein, Y.S.; Soliman, S.H.; Saad, M.F.; Eliraqy, Entesar Z.

doi:10.21608/javs.2022.141246.1152

	Cysteine enhance Holstein bull’s sperm assessment quality and conception rate when added to duck egg yolk low-density lipoproteins
Journal of Applied Veterinary Sciences
Article 12, Volume 7, Issue 3, July 2022, Page 86-93 PDF (572.91 K)
Document Type: Original Article
DOI: 10.21608/javs.2022.141246.1152
View on SCiNiTO
Authors
Y.S. Hussein ¹; S.H. Soliman²; M.F. Saad²; Entesar Z. Eliraqy²
¹Biotechnology Research Department, Animal Production Research Institute, Agricultural Research Center, Egypt
²Animal Production Research Institute, Agricultural Research Center, Egypt
Abstract
Spermatozoa undergo a significant transformation during the cryopreservation process, including oxidative stressors during dilution, cooling, equilibration, or freezing and thawing stages that postpone their motility and viability. When duck egg yolk is added to low-density lipoproteins (LDL), which has a considerable cryoprotective effect, the sperm is successfully protected from cold shock and the lipid-phase transition during the freeze-thaw process. The amino acid cysteine, on the other hand, functions as a precursor of intracellular glutathione (GSH). The purpose of the current study was to examine the effects of employing 10 percent LDL duck egg yolk, with or without cysteine, for Holstein bull spermatozoa and to use cryopreserved straws in fertility trials to verify the findings. According to experimental findings, 10mM cysteine applied to 10% LDL duck.
Keywords
Cysteine; Duck egg Yolk; Holstein’s Semen; LDL


References
ALAMAARY, M. S., HARON, A.W., HIEW, M.W. H., and ALI, M., 2020. Effects of cysteine and ascorbic acid in freezing extender on sperm characteristics and level of enzymes in post-thawed stallion semen. Vet Med Sci., 6:666-672. https://doi.org/10.1002/vms3.315 AL-AMERI, M.H., MOHAMMED, A. J., ABDUL-RAHAMAN, Y. T., and ABD AL-RAZAQ, M. T., 2020. Effect of addition of cysteine and tryptophan to diluted and cooled semen of awassi rams. Plant Archives, Vol. 20 No. 2, pp. 6515-6520. https://publons.com/researcher/4423603/maad-hasani-al-ameri AMIRAT, L., ANTON, M., TAINTURIER, D., CHATAGNON, G., BATTUT, I., and COURTENS, J. L., 2005. Modifications of bull spermatozoa induced by three extenders: Biociphos, low density lipoprotein and Triladyl, before, during and after freezing and thawing. Reproduction, 129, pp. 535–543. DOI: 10.1530/rep.1.00011 ANAND, M. S. YADAV, and SHUKLA, P., 2014. Cryoprotectant in semen extender: From egg yolk to low-density lipoprotein (LDL). Livestock Research International, Vol 2, Issue 3 Pages 48-53. DOI: 10.15740/HAS/TAJAS/10.2/00-00 ANSARIA, M. S., RAKHAB, B. A., MALIKA, M. F., ANDRABIC, S. M. H., ULLAHC, N., IQBALA, R., HOLTD, W. V., and AKHTER, S., 2016. Effect of cysteine addition to the freezing extender on the progressive motility, viability, plasma membrane and DNA integrity of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa. Journal of Applied Animal Research. https://doi.org/10.1080/09712119.2014.987292 BALLESTER, J., JOHANNISSON, A., SARAVIA, F., and HÅÅRD, M., 2007. Post-thaw viability of bull AI-doses with low-sperm numbers. Theriogenology 68(6):934-43. DOI: 10.1016/j.theriogenology.2007.07.008 BATHGATE, R., MAXWELL, W.M.C., and EVANS, G., 2006. Studies on the effect of supplementing boar semen cryopreservation media with different avian egg yolk types on in vitro post-thaw sperm quality. Reproduction in Domestic Animals, 41, 68-73. DOI: 10.1111/j.1439-0531.2006.00623. BERGERON, A., and MANJUNATH, P., 2006. New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Molecular Reproduction and Development, 73: 1338-1344. DOI: 10.1002/mrd.20565. BILODEAU, J.F., BLANCHETTE, S., GAGNON, I. C., and SIRARD, M. A., 2001. Thiols prevent H2O2-mediated loss of sperm motility in cryopreserved bull semen. Theriogenology, 56: 275- 286. DOI: 10.1016/s0093-691x(01)00562-3 BUCAK, M. N., ATESSAHIN, A. and YUCE, A., 2008. Effect of antioxidant and oxidative stress parameters on ram semen after the freeze-thawing process. Small Ruminant Research, 75: 128-134. https://doi.org/10.1016/j.smallrumres.2007.09.002 BUCAK, M.N., and UYSAL, O., 2008. The role of antioxidants in freezing of Saanen goat semen. Indian Vet J. 85: 148-150. DUNCAN, D.B. 1955. Multiple Range and Multiple F. Test Biometrics.,Biometrics. 11: 1-42. https://doi.org/10.2307/3001478 EL-BADRY, D.A., RAWASH, Z.M., ABD ELAAL, M., and ALI, A. H., 2015. The Effect of LDL from Different Bird’s Eggs on the Freezability of Buffalo Spermatozoa. Global Veterinaria 14 (2): 158-165. DOI:10.5829/idosi.gv.2015.14.02.92111 EL-SHARAWY, M.E., EL-SEIFY, M. E., HUSSIEN, A. M., EL-SHERBIENY, M., and EL-SHAMAA, I. S., 2015. Effect of Duck Egg Yolk on Cryopreservation and Fertility of Egyptian Buffalo Bull Semen. International Journal of Agriculture Innovations and Research Volume 3, Issue 4, ISSN (Online) 2319-1473. EL-SHESHTAWY, R.I., EL-SISY, G.A., and EL-NATTAT, W.S., 2008. Use of Selected Amino Acids to Improve Buffalo Bull Semen Cryopreservation. Global Veterinaria 2 (4): 146-150, GUALTIERI, R., BARBATO, V., FIORENTINO, I., and BRAUN, S., 2014. Treatment with zinc, D-aspartate, and coenzyme Q10 protects bull sperm against damage and improves their ability to support embryo development Theriogenology 82(4). DOI: 10.1016/j.theriogenology. 2014.05.028 HANCOCK,J. L. 1951, A Staining Technique for the Study of Temperature-Shock in Semen. Nature vol. 167, 323–324. DOI: 10.1038/167323b0 https://scirp.org/reference/referencespapers.aspx?referenceid=1384182 HUSSEIN, Y. S. 2018. Cryoprotective efficacy of local friesian bull’s spermatozoa using tris-extender enriched with natural or synthetic antioxidants. Egyptian J. Anim. Prod. 55(1):1-6. JEYENDRAN, R. S., VAN DER VEN, H. H.B., PEREZ-PELAEZ, M., and CRABO, BO.G., 1984. Development of an assay to assess the functional integrity of human sperm membrane and its ralationship to other characteristcs. J Reprod Fertil 70 (1):219-28. DOI: 10.1530/jrf.0.0700219. KHALILI, B., JAFAROGHLI1, M., FARSHAD, A., and PARESH-KHIAVI, M., 2010. The Effects of Different Concentrations of Glycine and Cysteine on the Freezability of Moghani Ram Spermatozoa. Asian-Aust. J. Anim. Sci. Vol. 23, No. 3: 318 – 325. DOI:10.5713/ajas.2010.90387 KHAN, I. M, XU, D., CAO, Z., LIU, H., KHAN, A., UR RAHMAN, S., AHMED, J. Z., RAHEEM, M. A., and ZHANG, Y., 2021. Addition of L-Cysteine and Vitamin E to Semen Diluent Enhances Freeze-thawed Spermatozoa Characteristics in Crossbred Cattle Bulls under Subtropical Environment. Pakistan J. Zool., vol. 53(4), pp 1309-1319. DOI: https://dx.doi.org/10.17582/journal.pjz/20191006091046 KIM, J.H., JANG, H.J., CH, W.Y., YEON, S.J., and LEE, C.H., 2020. In vitro antioxidant actions of sulfur-containing amino acids. Arabian J. Chem., 13: 1678-1684. https://doi.org/10.1016/j.arabjc.2017.12.036 KOOHESTANIDEHAGHI, Y., TORKAMANPARI, M., SHIRMOHAMADI, Z., LORIAN, K., and VATANKHAH, M., 2020. The effect of cysteine and glutamine on human sperm functional parameters during vitrification. Andrologia; 53:e13870. DOI: 10.1111/and.13870. LODHI, L.A., ZUBAIR, M., QURESHI, Z. I., AHMAD, I., and JAMIL, H., 2008. Correlation between hypo-osmotic swelling test and various conventional semen evaluation parameters in fresh Nili-Ravi Buffalo and Sahiwal cow bull semen. Pakistan Vet J 28:186-188. https://agris.fao.org/agris-search/search.do?recordID=DJ2012040464 MANJUNATH, P. 2012. New insights into the understanding of the mechanism of sperm protection by extender components. Animal Reproduction, 9(4): 809-815. MEMON, A. A., WAHIDA, H., ROSNINAA, Y., GOHB, Y.M., EBRAHIMI, M. B., NADIAC, F. M., and AUDREYC, G., 2015. Effect of hypotaurine and cysteine on sperm cytological parameters of cooled and post thaw boer goat semen. Elixir Semen Dilu. & Freez. 79, 30196-30200. https://www.elixirpublishers.com/articles/1422707484_79%20(2015)%2030196-30200.pdf MORRIS, G.J., and CLARK, A., 1987. The effects of law temperatures on biological systems. London: Edward Arnold. Pg 72-119. DOI:10.3184/003685003783238734 MOUSSA, M, MARTINET, V., TRIMECHE, A., TAINTURIER, D., and ANTON, M., 2002. Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenology, 57: 1695-1706. DOI: 10.1016/s0093-691x(02)00682-9 PERUMA, P. 2018. Low density lipoprotein in cryopreservation of semen. Asian Pacific Journal of Reproduction. 103-116 SAS, 1999. Statistical Analysis System SAS User’s Guide Statistics. SAS Institute Inc. Editors, Cary, NC. TARIQ, M., KHAN, M.S., SHAH, M.G., NISHA, A.R., UMER, M., HASAN, S.M., RAHMAN, A. and RABBANI, I., 2015. Exogenous antioxidants inclusion during semen cryopreservation of farm animals. J. Chem. Pharm. Res., 7(3): 2273-2280. DOI: 20153176322 TUNCER, P. B, SARIÖZKAN, S., BUCAK, M. N., and BÜYÜKLEBLEBICI1, S., 2021. Antioxidant supplementation ameliorates bull sperm parameters and fertilizing ability following the freeze-thaw process. Turk J Vet Anim Sci, 45: 457-462. DOI:10.3906/vet-2004-88. UYSAL, O., and BUCAK, M. N., 2007. Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. ActaVeterinaria Brno, 76: 383–390. DOI:10.2754/avb200776030383 VASAN, S. S. 2011. Semen analysis and sperm function tests: How much to test? Indian J Urol.; 27(1): 41–48. doi: 10.4103/0970-1591.78424 WATSON, P. F. 1975. Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet Rec. 5;97(1):12-5. DOI: 10.1136/vr.97.1.12 WATSON, P.F. 1981. The roles of lipid and protein in the protection of ram spermatozoa at 5 degrees C by egg-yolk lipoprotein. Journal of Reproduction and Fertility, 62: 483-492. DOI: 10.1530/jrf.0.0620483. ZHU, Z., ZENG, Y., and ZENG, W., 2022. Cysteine improves boar sperm quality via glutathione biosynthesis during the liquid storage. Anim Biosci Vol. 35, No. 2:166-176. DOI: 10.5713/ab.21.0151.
Statistics Article View: 292 PDF Download: 348