EFFECT OF PROPAGATION METHOD AND NITROGEN FERTILIZATION ON STEVIA (STEVIA REBAUDIANA. BERTONI) YIELD AND QUALITY IN EGYPT | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 16, Volume 83, Issue 3, September 2005, Page 1269-1291 PDF (661.08 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2005.247597 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| AHMED E. ATTIA1; OLFAT E. EL-BAGOURy2; ABDEL-WAHAB I. ALLAM1; ASHRAF M. ABDEL-GHANY2 | ||||
| 1Sugar Crops Research Institute, Agricultural Research Center, Giza, Egypt | ||||
| 2Department of Agronomy, Faculty of Agriculture, Ain Shams University, Cairo, Egypt | ||||
| Abstract | ||||
| Two field experiments were carried out in Giza Agricultural Station - Agricultural Research Center during the two successive seasons 2001j2002 and 2002j2003 to study the effect of propagation method (seed, tissue culture and root cuttings), nitrogen fertilization (0, 20 and 40 kg Nlfedlcut) and their interaction on Stevia rebaudiana yield and quality. Seeds of stevia variety Spanti imported from Spain and locally regenerated vegetatively were used to produce plantlets, root cuttings and shoot apex tissue culture. Results showed that tissue culture and root cuttings propagated plants were significantly taller than seed propagated plants. Tissue culture and root cutting propagation methods exceeded seed propagated plants in No. of branches j plant in first, second and fifth cuts. Root cutting propagated plants gave the maximum values of leaves fresh weight (2.7, 3.12, 1.55 and 1.58 t/fed for the first, second, third and fourth cuts, respectively). Plants propagated through root cuttings exceeded plants propagated by seed and tissue culture in stevioside % in all cutting dates giving maximum value of 24.16% in the first season and 21.14% in the second season in the 5th cut respectively. Application of 40kg N/fed/cut gave significant maximum values of plant height in three cuts (54.06, 54.33 and 53.07cm. for first, second and fifth cuts, respectively). Nitrogen fertilization affected all cuts for the No. of branchesjplant and the application of either 20 or 40 kg N/fed/cut significantly exceeded 0 kg N/fed/cut. Application of 40 kg N/fed/cut gave maximum values of fresh leaves weight in all cutting dates (2.27, 2.86, 1.56, 1.56 and 3.37 tjfed respectively), meanwhile dry leaves weight yield gave (0.87, 0.96, 0.52, 0.52 and 1.13 tjfed. respectively). Application of 20 and 40 kg N/fedcut exceeded Okg N/fedlcut in stevioside % in both seasons. Application of 40 kg Nlfed/cut for stevia plants propagated by root cutting produced maximum yield of dry leaves weights of 1.06, 1.13 and 0.54 t/fed. for first, second and fourth cuts, respectively. Therefore, total stevioside yield reached its maximum value (962.43 kg/fed/year) when root cutting propagation method and application of 40 kg N/fed/cut were used. | ||||
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