PRODUCTION OF CELLULOLYTIC ENZYMES BY Trichoderma viride GROWN ON SUGAR BEET INDUSTRIAL WASTES | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 28, Issue 12, December 2003, Page 8407-8417 PDF (492.31 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2003.247786 | ||||
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| Authors | ||||
| A. E. Selim,; H. A. EI-Fadaly; Aida H. Afify; Amal A. Abd·El-Rahman | ||||
| Microbiol. Oept.; Fac. Agric., Mansoura Univ., Mansoura, Egypt. | ||||
| Abstract | ||||
| Four fungal strains were grown using sugar beet industrial by-produJs. These wastes were sugar beet pulp. sugar beet leaves, sugar beet molasses as low cost cameo source. The optimization for the enzymatic production was examinfd such as growth temperature, initial pH of fungal growth and the effect of nitrogen source. Some properties of cellulases cbtained by Trichoderma vino« were al~o studied such as temperature, pH, Incubation lime and thermal stability of Ire enzymatic activity. Cellulases activities were found at the maximum point aller the 6th day of shake flask cultivation for the three tested enzymes, FP-ase, CMC-ase and Cb- ase by Aspergillus niger. In case of Aspergillus fumigatus data showed Ihe same behaviour except CMC-ase activity which produced after 9th day of fermentation where 8 mixture of leaves and molasses was used as carbon SOlJrce/ For Trichoderma viride, sugar beet pulp gave the maximum enzymatic production of both CMC-ase and C.b-ase after the 6th day of fermentation, while FP-ase was reduced atler that lime. Using beet leaves for growing Trichoderma hanianl1m, both FP-afe and C.b-ase were in their optimal production while CMC-asel reached its peak when the fungus grown on sugar beet leaves with molases In 1:1 ratio. The growth temperature of ~O·C and pH 5 were the optimum for the selected fungal strain. Trichoderm8 viride for the three tested cellutases. The Cultivation medium containing peptone and (NH.)2S0. as N source showed to be the best for the production of the three tested cellulases. For the cellulolytic aclivities, the optimum temperature and pH were 60·C and 5.5, respectively. For the incubation time, 18hr, 1 hr and 45 mIn, proved to be the besl for FP·ase, CMC-ase and Case, respectillely. The thermal stabilily of the cellulases was remained to 70·C. | ||||
| Keywords | ||||
| Cellutsses; Fermentation; Environmental pollution; Sugar beet by-product; Trichoderm8 viride | ||||
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