Zoonotic importance of Vancomycin-resistant Enterococcus faecalis. | ||||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||||
| Articles in Press, Accepted Manuscript, Available Online from 04 July 2022 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/scvmj.2022.146826.1088 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Zeinab Said Ahmed  1; Mona Kadry2; Fatma Abdel-kader3
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| 1Zoonoses department. faculty of veterinary medicine Cairo university | ||||
| 2Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University. | ||||
| 3Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University | ||||
| Abstract | ||||
| A worst-case scenario for nosocomial infections led to the rise of vancomycin-resistant enterococci (VRE). This study assesses the occurrence of Enterococcus faecalis in 200 feacal samples and 133 milk samples which collected from cattle and buffalo. About 70 human samples were collected as stool sample and hand swabs from animal clinics and farms. Isolation and identification of the bacteria were performed using conventional cultural techniques, biochemical identification and were confirmed by PCR amplification. Antimicrobial susceptibility against six antimicrobial agents commonly used in human and animals was tested using the disc diffusion method. The resistance of E.faecalis to vancomycin (VAN) was confirmed by PCR targeting the vanA and vanB genes. The occurence of E.faecalis in cattle and buffaloe were 8.1% and 15.5% Respectively. Normal milk sample showed 12.8% of E.faecalis while abnormal milk samples were 37.5%. E.faecalis infection was recorded as 25.7% in workers contact. Antimicrobial resistant to Vancomycin was 11.1% ,14.2%, 10.5% and 50% in cattle, buffalo, milk and human respectively. Resistance genes (van A and van B) were detected in all Vancomycin resistant strains. This study showed that E. faecalis which is vancomycin resistant can be discovered not only in milk and animal products but also in human contact, which is a matter of public health. | ||||
| Keywords | ||||
| E.faecalis; vancomycin-resistant; human; milk | ||||
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