Swift and Enhanced-Sensitive Analytical Method for Determination of Fumonisins FB1 And FB2 in Egyptian Oilseeds Using LC-MS/MS. | ||||
| Egyptian Journal of Chemistry | ||||
| Article 49, Volume 65, Issue 12, December 2022, Page 543-554 PDF (526.59 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2022.129939.5730 | ||||
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| Authors | ||||
atef ahmed badr  1; Hamdy Ali2; Ahmed Abd El-Hakim2; Ahmed Mamdouh Gomaa3
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| 1QCAP | ||||
| 2Department of Biochemistry, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt | ||||
| 3Head of Dep., Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Food | ||||
| Abstract | ||||
| Fumonisins (FBs) are harmful carcinogenic natural toxins produced by fungi from Fusarium species whereas found in oilseeds crops. Fumonisin B1 (FB1) and Fumonisin B2 (FB2) are the most serious natural food contaminants among the several known Fumonisin. The main purpose of this study is to establish an accredited rapid and ultra-sensitive method for the investigation of FB1 and FB2 in Egyptian oilseed. LC-MS/MS with the positive-ion electrospray ionization (+ESI) mode has been employed for this investigation. The limits of quantification (LOQ) were 3.9 µg/Kg for FB1 and 2.3 µg/Kg for FB2. The limits of detection (LOD) were 1.2, and 0.7 µg/Kg for FB1 and FB2 respectively with 21% expanded uncertainty. The test method has been used to review six governorates of Egypt on corn, white corn, soybean, sesame, and sunflower seeds. For corn, the contamination levels of total FB1 and FB2 were varied between <LOQ to 10034.9 µg/Kg, while in white corn was from <LOQ to 7571 µg/Kg. The total FB1 and FB2 were from <LOQ to 321.9 µg/Kg in soybean samples. whereas sesame and sunflower seeds were devoid of FB1 and FB2. | ||||
| Keywords | ||||
| Fumonisin; FB1; FB2; LC-MS/MS; method determination; Egyptian oilseed | ||||
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