The Possible Protective Role of Vitamin C against Bisphenol-A-Induced Structural Changes on the Adrenal Cortex of Adult Male Albino Rat: Histological, Immunohistochemical and Morphometric Study | ||||
| Egyptian Journal of Histology | ||||
| Article 29, Volume 46, Issue 3, September 2023, Page 1418-1430 PDF (7.41 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2022.132167.1670 | ||||
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| Authors | ||||
Reneah Refaat Bushra   1; Hala Mohamed Hassanin2
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| 1department of human anatomy and embryology, faculty of medicine, Assiut University,Assiut,Egypt | ||||
| 2Department of human anatomy and embryology, Faculty of Medicine,Assiut University | ||||
| Abstract | ||||
| Introduction: Bisphenol-A is a monomer in epoxy resins and polycarbonate plastics synthesis. However, it is an endocrine disruptor participating in several adverse health problems. Vitamin C is a potent antioxidant. Aim of the Study: This study aimed to detect the effects of Bisphenol-A on the structure of the adrenal cortex and the possible impact of concomitant administration of vitamin C in protection against these changes in the adult male albino rat. Material and Methods: 45 adult male albino rats weighing (200-250) gm were used. The rats were randomly divided into three equal groups. Group 1: control group was given distilled water for six weeks. Group 2: received 25 mg BPA /kg/day, once daily orally, for six weeks. Group 3: received 25 mg BPA /kg/day and 60 mg vitamin C /kg/ day, once daily orally for six weeks. At the end of the experiment, the rats were sacrificed. The left adrenals were removed, and processed for light and electron microscopic, immunohistochemical and morphometric studies. Results: Bisphenol-A intake caused structural changes in the adrenal cortex, such as loss of normal arrangement of cells, congestion of blood capillaries, vacuolization of cytoplasm, nuclear pyknosis, shrinkage of the nuclei, thickening of the capsule, strong positive caspase-3 cytoplasmic reaction, marked significant reduction of the thickness and cell diameter of all three layers, and increase of the %Area of caspase-3 immunoreactivity. Vitamin C cancelled most of these light and electron histological, immunohistochemical, and morphometric effects. Conclusion: Bisphenol-A administration has damaging effects on the adrenal cortex and vitamin C protects against these effects. | ||||
| Keywords | ||||
| Adrenal cortex; albino rat; bisphenol-A; vitamin C | ||||
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