Phytochemical Profile of Phoenix rupicola T. Anderson Seeds and In Vitro Evaluation of their Estrogenic Activity using MCF-7 Cell Lines | ||||
Egyptian Journal of Chemistry | ||||
Volume 65, Issue 132, December 2022, Page 1457-1464 PDF (1011.22 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejchem.2022.129433.5731 | ||||
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Authors | ||||
Enaam AbouZeid 1; Rehab Hussein2; Ahmed Afifi3; Abeer Salama 4; Fadia Youssef5; Sherweit El-Ahmady6; Nagwa Ammar7 | ||||
1Department of Pharmacognosy, Pharmaceutical and Drug Indutsries Research Institute, National Research Centre, Cairo, Egypt | ||||
2Pharmacognosy Department, National Research Centre, Dokki, Cairo, 12622, Egypt | ||||
3bPharmacognosy Department, Pharmaceutical and Drug Industries Research Division, National Research Centre | ||||
4Department of Pharmacology, National Research Centre | ||||
5Pharmacognosy Department, Faculty of Pharmacy, Ain Shams University. Cario, Egypt | ||||
6Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams Univeristy | ||||
7Department of Pharmacognosy, Pharmaceutical and Drug Industries Research Institute, National Research Centre | ||||
Abstract | ||||
The current study aimed to investigate the phytochemical profile and the in vitro estrogenic activity of Phoenix rupicola T. Anderson seeds’ extracts. Gas chromatography/ mass spectrometry (GC/MS) of the petroleum ether (seed oil) and chloroform extracts revealed the characterization of 93 compounds. γ-Sitosterol (8.54%), n-dodecane (6.00%), n-undecane (5.87%) and (2E)-2-decenal (3.48%) represented the major compounds in the petroleum ether extract, whereas lauric acid (21.73%), γ-sitosterol (21.21%), palmitic acid (9.19%) and myristic acid (6.37%) were the main components in the chloroform extract. Moreover, investigation of the 70% aqueous methanol extract by high-performance liquid chromatography coupled with diode-array detector (HPLC-DAD) revealed the identification of 13 phenolic compounds, of which catechin (105.75 mg%), vanillic acid (58.26%), p-hydroxybenzoic acid (32.84%) and cinnamic acid (27.62%) dominated The estrogenic activity of the extracts was tested in vitro via sulforhodamine B (SRB) assay on MCF-7 cells, where petroleum ether extract at a dose of 10 ug/mL exhibited the highest activity (p < 0.05) indicated by 1.17 % increase in cells’ proliferation compared to 17β-estradiol. Consequently, P. rupicola seeds provide a promising source of phytoconstituents with estrogenic activity worthy for further detailed studies. | ||||
Keywords | ||||
Phoenix rupicola seeds; GC/MS; HPLC-DAD; in vitro estrogenic assay; MCF-7 cell lines | ||||
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