Production of PHAs from Waste Frying Oil by Pseudomonas fluorescens S48 Using Different Bioreactor Feeding Strategies | ||||
| Egyptian Journal of Microbiology | ||||
| Article 1, Volume 47, Issue 1, December 2012, Page 1-15 PDF (792.64 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejm.2012.249 | ||||
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| Abstract | ||||
| BBIODEGRADABLE polyesters, polyhydroxyalkanoate (PHAs) are promising candidates for the development of environment-friendly and totally biodegradable plastics. The production of PHAs by Pseudomonas fluorescens S48 was examined using bioreactor as one-stage batch, two-stage batch, and fed-batch (pulsed, continuous and high cell density) cultures. Corn oil , soybean oil (extracted from their meal) and two types of waste frying oils (WFO) were used in the productive medium as a carbon source. The highest values of polymer content in the one-stage bioreactor fermentation (52 % and 76.53 %) were obtained after 60 h on media supplemented with extracted corn oil and soybean oil, respectively. Whereas, the highest figure obtained on WFO type 2 was 30 % under the same circumstances. Using bioreactor as a two-stage batch culture increased the polymer content with soybean, corn oil and WFO type 2 by 2.2 %, 32.11 % and 58 %, respectively as compared with that obtained in one-stage batch culture. High-cell-density (0.64 gl-1) at continuous feeding rate 0.55mll-1h-1 of WFO type 2 recorded the highest polymer content, yield and conversion coefficient after 48 h. Moreover, this application increased the polymer content by 65.7 % than that obtained in onestage batch culture as well as reduced the fermentation period 12 hr | ||||
| Keywords | ||||
| Pseudomonas fluorescens S48; PHAs; Batch; Two-stage batch; High-cell-density; Bioreactor | ||||
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