Characterization of rhamnolipids produced by Pseudomonas putida ON763757 isolated from petroleum contaminated soils
Tawila, S., Elmeihy, R., Youssef, A., Zaghloul, R., Abou-Aly, H. (2022). Characterization of rhamnolipids produced by Pseudomonas putida ON763757 isolated from petroleum contaminated soils. EKB Journal Management System, 65(132), 759-767. doi: 10.21608/ejchem.2022.148123.6406
Samaa, Tawila; Rasha M. Elmeihy; Ahmed Youssef; Rashed A. Zaghloul; Hamed E. Abou-Aly. "Characterization of rhamnolipids produced by Pseudomonas putida ON763757 isolated from petroleum contaminated soils". EKB Journal Management System, 65, 132, 2022, 759-767. doi: 10.21608/ejchem.2022.148123.6406
Tawila, S., Elmeihy, R., Youssef, A., Zaghloul, R., Abou-Aly, H. (2022). 'Characterization of rhamnolipids produced by Pseudomonas putida ON763757 isolated from petroleum contaminated soils', EKB Journal Management System, 65(132), pp. 759-767. doi: 10.21608/ejchem.2022.148123.6406
Tawila, S., Elmeihy, R., Youssef, A., Zaghloul, R., Abou-Aly, H. Characterization of rhamnolipids produced by Pseudomonas putida ON763757 isolated from petroleum contaminated soils. EKB Journal Management System, 2022; 65(132): 759-767. doi: 10.21608/ejchem.2022.148123.6406

Characterization of rhamnolipids produced by Pseudomonas putida ON763757 isolated from petroleum contaminated soils

Egyptian Journal of Chemistry
Volume 65, Issue 132, December 2022, Page 759-767  XML PDF (777 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.148123.6406
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Authors
Samaa, Tawila1; Rasha M. Elmeihyorcid 2; Ahmed Youssef email 3; Rashed A. Zaghloul4; Hamed E. Abou-Aly4
11Department of Agricultural Microbiology, Faculty of Agriculture, Benha University, Moshtohor, Kaluybia, 13736, Egypt;
2Faculty of Agriculture, Benha University, Egypt
3National Research Centre, 30 El-Tahrir Street
4Agricultural Microbiology Department, Faculty of Agriculture, Benha University, Moshtohor, Kaluybia, 13736, Egypt.
Abstract
Sixteen biosurfactant-producing bacteria were isolated from multiple petroleum-contaminated soils in Kaluybia Governorate, Egypt. Six screening tests (emulsification index (EI24%), oil spreading assay, emulsification activity, cetyltrimethylammonium bromide (CTAB), hemolytic and lipolytic activities) were conducted to select the high producer isolate. Out of 16 isolates, only one was chosen for molecular identification as the most efficient biosurfactant-producer and was found to be Pseudomonas putida ON763757. The type of biosurfactant was determine via three chromatography analytical methods, High-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR) and Gas chromatography–mass spectrometry analysis (GC-MS). HPLC analysis showed that chlorogenic acid (1409.20 µgg-1) was the main component, followed by ellagic acid (530.12 µgg-1). FTIR and GC-MS analyses indicated that the biosurfactant of Ps. putida was rhamnolipid, which is an exciting product for environmental and industrial applications.
Keywords
Biosurfactants; Emulsification; Chromatographic Technique; Pseudomonas putida; Rhamnolipid
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