Effect of Aqueous and Methanolic Green Tea Extracts on Motility, Normal Forms, And Kinetic Parameters of Spermatozoa in Rahmani Ram Semen Equilibrated at 5 °C for 4 Hours | ||||
Journal of Animal and Poultry Production | ||||
Article 2, Volume 13, Issue 5, May 2022, Page 63-70 PDF (978.08 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jappmu.2022.145028.1041 | ||||
View on SCiNiTO | ||||
Authors | ||||
M. A. Abdellatif1; S. A. Gabr1; M. A. El-Sherbieny2; A. I. Yousif 3 | ||||
1Animal Production Department, Faculty of Agriculture, Tanta University, Egypt. | ||||
2Animal Production Research Institute, Agricultural Research Center, Egypt. | ||||
3Animal Production Research Institute, Agricultural Research Center, Cairo, Egypt. | ||||
Abstract | ||||
Green tea extract is plant–derived natural antioxidant. The purpose of this study was to investigate the effect of the supplementing semen extender (Tris-citric-soybean lecithin) with different levels of aqueous (AGTE) or methanolic (MGTE) green tea extract (0.5, 1.0 and 1.5%) on the motility, livability, normality, plasma membrane integrity, and kinetic parameters of ram spermatozoa in diluted and equilibrated semen. Semen was collected from 5 rams once a week for 7 weeks using an artificial vagina. Results showed positive effect of extender containing 1% MGTE on visual motility, livability, abnormality, membrane integrity, rapid motility, sperm velocity, and normal form in post-equilibrated semen. It is possible to infer that the inclusion of methanolic green tea extract at a level of 1% in semen extender had a substantial effect on sperm quality, normality, function, and velocity of ram spermatozoa in Rahmani semen after dilution and equilibration for 4 h at 5oC. | ||||
Keywords | ||||
Aqueous and Methanolic green tea extract; ram spermatozoa; equilibration; motility CASA analysis | ||||
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