PCR Versus Cytotoxicity Assay for Diagnosis of Antibiotics Associated Diarrhea Caused by Clostridium difficile infection
Abdel Khalek, H., Ramadan, M., Abedlhamid, A. (2020). PCR Versus Cytotoxicity Assay for Diagnosis of Antibiotics Associated Diarrhea Caused by Clostridium difficile infection. EKB Journal Management System, 29(1), 51-54. doi: 10.21608/ejmm.2020.249856
Hanan S. Abdel Khalek; Mona O. Ramadan; Amir F. Abedlhamid. "PCR Versus Cytotoxicity Assay for Diagnosis of Antibiotics Associated Diarrhea Caused by Clostridium difficile infection". EKB Journal Management System, 29, 1, 2020, 51-54. doi: 10.21608/ejmm.2020.249856
Abdel Khalek, H., Ramadan, M., Abedlhamid, A. (2020). 'PCR Versus Cytotoxicity Assay for Diagnosis of Antibiotics Associated Diarrhea Caused by Clostridium difficile infection', EKB Journal Management System, 29(1), pp. 51-54. doi: 10.21608/ejmm.2020.249856
Abdel Khalek, H., Ramadan, M., Abedlhamid, A. PCR Versus Cytotoxicity Assay for Diagnosis of Antibiotics Associated Diarrhea Caused by Clostridium difficile infection. EKB Journal Management System, 2020; 29(1): 51-54. doi: 10.21608/ejmm.2020.249856

PCR Versus Cytotoxicity Assay for Diagnosis of Antibiotics Associated Diarrhea Caused by Clostridium difficile infection

Egyptian Journal of Medical Microbiology
Volume 29, Issue 1, January 2020, Page 51-54  XML PDF (275.53 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2020.249856
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Authors
Hanan S. Abdel Khalek* 1; Mona O. Ramadan1; Amir F. Abedlhamid2
1Medical Microbiology and Immunology Department Faculty of Medicine Tanta University
2General Surgery Department, Tanta University Hospital
Abstract
Background: Antibiotic Associated Diarrhea (AAD) is a common health problem in patients under antibiotic therapy. Clostridium difficile is the commonest cause of AAD. Objectives: our study aimed to compare between cytotoxicity assay and PCR for the diagnosis of Clostridium difficile antibiotic associated diarrhea as regard specificity and sensitivityMethodology: Stool samples were collected from patients clinically suspected to have antibiotic associated diarrhea and proved by sigmoidscope from General Surgery Department in Tanta University. All samples were subjected to cytotoxicity assay to detect C-difficile toxin and PCR to detect ctd B gene encoding for toxin B. Results: Cytotoxicity test showed specificity 96% and sensitivity 90.5%.while PCR assay showed specificity 100% and sensitivity 98%. Conclusion: PCR is rapid, specific and sensitive in the diagnosis of C-difficile Antibiotic associated diarrhea.
Keywords
Cytotoxicity; PCR; AAD
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