Activity of Fosfomycin against Extended-Spectrum-Beta-Lactamase producing Enterobacteriaceae isolates from Menoufia University Hospitals
Gharieb, M., Ghonaim, M., Makled, A., Sharweed, M. (2020). Activity of Fosfomycin against Extended-Spectrum-Beta-Lactamase producing Enterobacteriaceae isolates from Menoufia University Hospitals. EKB Journal Management System, 29(1), 111-119. doi: 10.21608/ejmm.2020.249865
Mohamed M. Gharieb; Mabrouk M. Ghonaim; Amal F. Makled; Moustafa M.A. Sharweed. "Activity of Fosfomycin against Extended-Spectrum-Beta-Lactamase producing Enterobacteriaceae isolates from Menoufia University Hospitals". EKB Journal Management System, 29, 1, 2020, 111-119. doi: 10.21608/ejmm.2020.249865
Gharieb, M., Ghonaim, M., Makled, A., Sharweed, M. (2020). 'Activity of Fosfomycin against Extended-Spectrum-Beta-Lactamase producing Enterobacteriaceae isolates from Menoufia University Hospitals', EKB Journal Management System, 29(1), pp. 111-119. doi: 10.21608/ejmm.2020.249865
Gharieb, M., Ghonaim, M., Makled, A., Sharweed, M. Activity of Fosfomycin against Extended-Spectrum-Beta-Lactamase producing Enterobacteriaceae isolates from Menoufia University Hospitals. EKB Journal Management System, 2020; 29(1): 111-119. doi: 10.21608/ejmm.2020.249865

Activity of Fosfomycin against Extended-Spectrum-Beta-Lactamase producing Enterobacteriaceae isolates from Menoufia University Hospitals

Egyptian Journal of Medical Microbiology
Volume 29, Issue 1, January 2020, Page 111-119  XML PDF (609.39 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2020.249865
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Authors
Mohamed M. Gharieb1; Mabrouk M. Ghonaim2; Amal F. Makled2; Moustafa M.A. Sharweed* 1
1Botany and Microbiology Department, Faculty of Science-Menoufia University
2Medical Microbiology and Immunology Department, Faculty of Medicine-Menoufia University
Abstract
Background: Fosfomycin is an old antibiotic that is being reconsidered for the treatment of lower urinary tract, due to the global increasing resistance of several bacteria to numerous-antimicrobials. Objectives: To evaluate the activity of fosfomycin and to detect fosfomycin resistant genes (FosA3 and FosC2) in extended-spectrum-beta-lactamase producing Enterobacteriaceae isolates from Menoufia University Hospitals. Methodology: The study included 155 samples collected from patients from different departments and ICUs at Menoufia University Hospitals, detected the ESBL-producing,by combined confirmatory method, fosfomycin resistance was assessed by disk diffusion and the minimal inhibitory concentration (MIC) by E-test method and fosfomycin resistance genes (FosA3 and FosC2) were detected by the conventional PCR. Results: Among the 48 ESBL-producing Enterobacteriaceae isolates analyzed in this study, 21 isolates had fosfomycin resistant genes (FosA3 and FosC2). Conclusion: Fosfomycin showed good antimicrobial activity against multidrug-resistant ESBL-positive Enterobacteriaceae. Resistance genes (FosA3 and FosC2) were detected in some strains, however, other resistance mechanisms to fosfomycin may exist.
Keywords
ESBL; fosfomycin; Enterobacteriaceae; Fos gene
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