Polymorphism of programmed death-1 (PD-1) gene in relation to susceptibility and progression of hepatitis B virus infection in Egyptian patients
Awad, S., El Gazzar, H., Allam, N., Elbahr, O., Abd El Aziz, A. (2020). Polymorphism of programmed death-1 (PD-1) gene in relation to susceptibility and progression of hepatitis B virus infection in Egyptian patients. EKB Journal Management System, 29(2), 55-62. doi: 10.21608/ejmm.2020.250018
Samah M. Awad; Hanaa M. El Gazzar; Naglaa Allam; Osama Elbahr; Azza M. Abd El Aziz. "Polymorphism of programmed death-1 (PD-1) gene in relation to susceptibility and progression of hepatitis B virus infection in Egyptian patients". EKB Journal Management System, 29, 2, 2020, 55-62. doi: 10.21608/ejmm.2020.250018
Awad, S., El Gazzar, H., Allam, N., Elbahr, O., Abd El Aziz, A. (2020). 'Polymorphism of programmed death-1 (PD-1) gene in relation to susceptibility and progression of hepatitis B virus infection in Egyptian patients', EKB Journal Management System, 29(2), pp. 55-62. doi: 10.21608/ejmm.2020.250018
Awad, S., El Gazzar, H., Allam, N., Elbahr, O., Abd El Aziz, A. Polymorphism of programmed death-1 (PD-1) gene in relation to susceptibility and progression of hepatitis B virus infection in Egyptian patients. EKB Journal Management System, 2020; 29(2): 55-62. doi: 10.21608/ejmm.2020.250018

Polymorphism of programmed death-1 (PD-1) gene in relation to susceptibility and progression of hepatitis B virus infection in Egyptian patients

Egyptian Journal of Medical Microbiology
Volume 29, Issue 2, April 2020, Page 55-62  XML PDF (430.41 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2020.250018
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Authors
Samah M. Awad* 1; Hanaa M. El Gazzar1; Naglaa Allam2; Osama Elbahr2; Azza M. Abd El Aziz1
1Department of Clinical Microbiology and Immunology and Molecular Microbiology in liver and Gastrointestinal Tract, National Liver Institute, Menoufia University, Menoufia, Egypt
2Depatment of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Menoufia, Egypt
Abstract
Background: chronic infection with hepatitis B virus (HBV) is a major risk factor for liver cirrhosis and hepatocellular carcinoma (HCC). Programmed death-1 (PD-1) has been involved in regulating immune responses to viral infections and tumors and has a critical role in HBV infection. Objectives: to investigate the effect of PD1 (+8669 G/A) single-nucleotide polymorphism (SNP) on disease susceptibility and progression of chronic HBV infection in Egyptian patients. Methodology: Blood samples were taken from 70 patients with confirmed chronic hepatitis B (CHB), HBV-related liver cirrhosis (LC) or HCC on top of HBV infection and 25 healthy controls. Genotyping of PD1 (+8669 G/A) polymorphism was studied using bidirectional PCR amplification of specific alleles (Bi-PASA). Results: A significantly higher frequency of PD-1 (+8669 G/A) AA genotype and A allele was found in CHB group (36.8 %) and LC group (21.7%) compared to control group (0.0%) (P< 0.05). On the other hand, the GG genotype and G allele were over represented in healthy controls (72.7%) than CHB group (52.6%) and LC group (47.8%). In assessing the risk of susceptibility to infection with HBV, The G allele was significantly predominant in healthy subjects compared to hepatitis B patients (89.4% versus 68.5%) (P< 0.05). G allele was markedly increased with disease progression to HCC (P = 0.006). Conclusion: Our study suggested that PD1 (+8669) polymorphism has significantly implicated in the disease susceptibility with AA genotype and A allele as a predisposing and the GG genotype and G allele as a preventive factors for chronic HBV infection. Also this study revealed a significant effect of the PD1 (+8669) polymorphism in the progression to HCC in hepatitis B patients.
Keywords
PD-1polymorphism; HBV; HCC
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