Role of circulating CD4+CD25+FOXP-3+T-regulatory cells and FOXP-3 rs3761548 gene polymorphism in acute liver allograft rejection
Ghoneim, E., Ghonaim, M., Makled, A., Altabbakh, M., Helwa, M., Salem, R., Awad, S. (2020). Role of circulating CD4+CD25+FOXP-3+T-regulatory cells and FOXP-3 rs3761548 gene polymorphism in acute liver allograft rejection. EKB Journal Management System, 29(2), 159-167. doi: 10.21608/ejmm.2020.250255
Enas M. Ghoneim; Mabrouk M. Ghonaim; Amal F. Makled; Mohamed A. Altabbakh; Mohamed Helwa; Radwa H. Salem; Samah M. Awad. "Role of circulating CD4+CD25+FOXP-3+T-regulatory cells and FOXP-3 rs3761548 gene polymorphism in acute liver allograft rejection". EKB Journal Management System, 29, 2, 2020, 159-167. doi: 10.21608/ejmm.2020.250255
Ghoneim, E., Ghonaim, M., Makled, A., Altabbakh, M., Helwa, M., Salem, R., Awad, S. (2020). 'Role of circulating CD4+CD25+FOXP-3+T-regulatory cells and FOXP-3 rs3761548 gene polymorphism in acute liver allograft rejection', EKB Journal Management System, 29(2), pp. 159-167. doi: 10.21608/ejmm.2020.250255
Ghoneim, E., Ghonaim, M., Makled, A., Altabbakh, M., Helwa, M., Salem, R., Awad, S. Role of circulating CD4+CD25+FOXP-3+T-regulatory cells and FOXP-3 rs3761548 gene polymorphism in acute liver allograft rejection. EKB Journal Management System, 2020; 29(2): 159-167. doi: 10.21608/ejmm.2020.250255

Role of circulating CD4+CD25+FOXP-3+T-regulatory cells and FOXP-3 rs3761548 gene polymorphism in acute liver allograft rejection

Egyptian Journal of Medical Microbiology
Volume 29, Issue 2, April 2020, Page 159-167  XML PDF (478.12 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2020.250255
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Authors
Enas M. Ghoneim1; Mabrouk M. Ghonaim2; Amal F. Makled2; Mohamed A. Altabbakh3; Mohamed Helwa4; Radwa H. Salem email 1; Samah M. Awad1
1Department of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University, Egypt
2Department of Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University, Egypt
3Department of Hepatology, National Liver Institute, Menoufia University, Egypt
4Department of Clinical Pathology, Faculty of Medicine, Menoufia University, Egypt
Abstract
Background: FOXP3 gene, is a major regulator of T-regulatory (Treg) cells that have a critical role in immune responses to alloantigens and acute cellular rejection (ACR) after liver transplantation (LT). Objectives: To investigate the role of FOXP-3 rs3761548 gene polymorphism on ACR after LT and to determine the relation between the circulating CD4+CD25+FOXP-3+Treg cells, and infection on the severity of ACR. Methodology: Blood samples were obtained from 60 allograft liver recipients and 20 healthy volunteers. FOXP-3 (rs3761548) polymorphism was studied using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Flowcytometry was used to enumerate circulating CD4+CD25+FOXP-3+Treg cells in peripheral blood of liver transplant recipients. Results: Bacterial and candida infections were more significantly detected in liver transplant recipients with ACR. A significant predominance of the AA genotype and the A allele of FOXP3 gene (rs3761548) was found in liver transplant recipients with compared to those without ACR. Highly significant reduction in percentage and count of CD4+and Treg cells were demonstrated in the liver transplant recipients with ACR. A significant negative correlation was detected between degree of ACR and percentage and count of Treg cells. Conclusion: Genetic polymorphism of FOXP-3 (rs3761548) and decreased percentage and count of Treg cells were associated with ACR. Carriers of the AA genotype and the A allele were more liable to severe degree of ACR after LT.
Keywords
FOXP-3 polymorphism; Treg cells; graft rejection; liver transplantation
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