Overcoming the Bottlenecks of Cellulose Utilization in Microbial Fuel Cells via Bioaugmentation Strategy with Cellulose‑Degrading Isolates
Khater, D., Amin, R., Zhran, M., Abd El-Aziz, Z., Hassan, H., Mahmoud, M., El-Khatib, K. (2023). Overcoming the Bottlenecks of Cellulose Utilization in Microbial Fuel Cells via Bioaugmentation Strategy with Cellulose‑Degrading Isolates. EKB Journal Management System, 66(3), 371-380. doi: 10.21608/ejchem.2022.138375.6095
Dena Z. Khater; R. S. Amin; M. O. Zhran; Zeinab K. Abd El-Aziz; Helmy M. Hassan; Mohamed Mahmoud; K. M. El-Khatib. "Overcoming the Bottlenecks of Cellulose Utilization in Microbial Fuel Cells via Bioaugmentation Strategy with Cellulose‑Degrading Isolates". EKB Journal Management System, 66, 3, 2023, 371-380. doi: 10.21608/ejchem.2022.138375.6095
Khater, D., Amin, R., Zhran, M., Abd El-Aziz, Z., Hassan, H., Mahmoud, M., El-Khatib, K. (2023). 'Overcoming the Bottlenecks of Cellulose Utilization in Microbial Fuel Cells via Bioaugmentation Strategy with Cellulose‑Degrading Isolates', EKB Journal Management System, 66(3), pp. 371-380. doi: 10.21608/ejchem.2022.138375.6095
Khater, D., Amin, R., Zhran, M., Abd El-Aziz, Z., Hassan, H., Mahmoud, M., El-Khatib, K. Overcoming the Bottlenecks of Cellulose Utilization in Microbial Fuel Cells via Bioaugmentation Strategy with Cellulose‑Degrading Isolates. EKB Journal Management System, 2023; 66(3): 371-380. doi: 10.21608/ejchem.2022.138375.6095

Overcoming the Bottlenecks of Cellulose Utilization in Microbial Fuel Cells via Bioaugmentation Strategy with Cellulose‑Degrading Isolates

Egyptian Journal of Chemistry
Volume 66, Issue 3, March 2023, Page 371-380  XML PDF (945.99 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.138375.6095
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Authors
Dena Z. Khater1; R. S. Amin1; M. O. Zhran2; Zeinab K. Abd El-Aziz2; Helmy M. Hassan3; Mohamed Mahmoudorcid 4; K. M. El-Khatib email orcid 5
1Chemical Engineering & Pilot Plant Department, Engineering and Renewable Energy Research Institute, National Research Centre, 33 El-Buhouth St., Dokki, Cairo 12311, Egypt.
2Faculty of Science (Girls), Botany and Microbiology Department, Al-Azhar University, Nasr City, Cairo, Egypt.
3Microbial Chemistry Department, National Research Centre, 33 El-Buhouth St., Dokki, Cairo 12311, Egypt.
4Water Pollution Research Department, National Research Centre, 33 El-Buhouth St., Dokki, Cairo 12311, Egypt.
5Chemical Engineering & Pilot Plant Department, Engineering and Renewable Energy Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Cairo 12311, Egypt.
Abstract
Although cellulosic biomass was among the most abundant substrates for producing renewable energy, its energy recovery in microbial fuel cells (MFCs) was often relatively low, owing to its low biodegradability. Here, the effect of bioaugmentation of three cellulose-degrading bacterial isolates (Acinetobacter tandoii, Brevundimonas bullata, and Micrococcus endophyticus) on MFCs efficiency was evaluated. Bioaugmented MFC with Acinetobacter tandoii strain showed the highest power densities (373 mW m-3) compared with non-bioaugmented MFC (240 mW m-3), associated with near-complete cellulose biodegradation. The results reveal that the bioaugmentation approach shaped the microbial community structure with the emergence of several phylotypes that were closely related to cellulose fermentation (Firmicutes and Bacteroidetes) and anode respiration (Proteobacteria), establishing a syntrophic partnership among cellulose-fermenting bacteria and electrochemically-active bacteria (EAB). In conclusion, our results confirm that the performance of mixed-culture MFCs fed with complex substrates (e.g., cellulose) could be improved by the bioaugmentation approach of fermenting isolates.
Keywords
Microbial Fuel Cell; Cellulose; Bioaugmentation; Microbial syntrophy; electrochemically active bacteria
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