PROTECTIVE EFFECT OF ASCORBIC ACID AND TIRON (4-5 DIHYDROXY- 1, 3 BENZENDISULFONIC ACID) AGAINST MERCURY CHLORIDEINDUCED OXIDATIVE STRESS AND NEUROTOXICITY IN RABBIT | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 11, Volume 54, Issue 117 - Serial Number 2, April 2008, Page 129-155 PDF (420.38 K) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.2008.251176 | ||||
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| Authors | ||||
| EMAN EZZ-ELDAWLA EL-SHARKAWY1; NEVEEN A. EL-NISR2 | ||||
| 1Dept. of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Assiut University | ||||
| 2Animal Health Institute, Assiut Branch, Assiut. | ||||
| Abstract | ||||
| The current study was performed to assess the potential of 4-5 dihydroxy-1, 3 benzendisulfonic acid (Tiron) and L-ascorbic acid (vitamin C) against inorganic mercury (mercuric chloride-HgCl2) induced oxidative stress and neurotoxicity in female New Zealand white rabbits. 10 rabbits per group were assigned to one of four treated groups: 0 mg HgCl2, 0 mg Ascorbic acid and 0 mg Tiron (control); 1mg Hg Cl2/kg BW orally; 1mg HgCl2/kg BW orally plus 1gm ascorbic acid /liter in drinking water; HgCl2/kg BW orally plus 471mg Tiron/kg BW I/P. Rabbits were administered HgCl2 and ascorbic acid for three months while Tiron administered in 6 concessive doses for 15 days at the level of three doses per week. Biochemical analyses on oxidative stress-related parameters and acetylcholine esterase activity as neurotransmitter were carried out. Histopathological analyses for detecting the cellular damage in brain tissues of exposed rabbits were also performed. Results obtained showed that HgCl2 significantly (p < 0.05) increased malondialdehyde and 4-hydroxyalkenals (MDA&4-HAE the marker of lipid oxidation) in brain tissues, while the activities of superoxide dismutase (SOD), Glutathione peroxidase (GSHPx) and acetylcholine esterase (AChE) activities were significantly (p < 0.05) decreased. Histopathological analysis of the brain revealed that neuronal degeneration with apoptotic features in cerebral cortex, hippocampus and cerebellum. Loss and significant (p < 0.05) decrease of purkinje cells number in cerebellum was detected. Also the purkinje cells lost the normal shape and became distorted. Most of the above parameters responded positively with either Tiron or vitamin C therapy, but more pronounced beneficial effects on the previous described parameters were observed in Tiron treated group. It is concluded that the protective effect of vitamin C as antioxidant and Tiron as a chelating agent against mercury chloride - induced neurotoxicity. Tiron was more effective than vitamin C in restoration of the most investigated parameters. | ||||
| Keywords | ||||
| Tiron; mercury chloride; purkinje cells; glutathione peroxidase | ||||
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