Phenotypic tests and Molecular detection of blaOXA-48 and blaNDM-1 genes among carbapenem-resistant Enterobacteriaceae isolated from allograft liver recipients
Awad, S., Ghoneim, E., Abd El Aziz, A., El Shaaraway, Y., Taha, M. (2020). Phenotypic tests and Molecular detection of blaOXA-48 and blaNDM-1 genes among carbapenem-resistant Enterobacteriaceae isolated from allograft liver recipients. EKB Journal Management System, 29(3), 29-36. doi: https://doi.org/10.51429/EJMM29304
Samah M. Awad; Enas M. Ghoneim; Azza M. Abd El Aziz; Yasmin A. El Shaaraway; Mohammad Taha. "Phenotypic tests and Molecular detection of blaOXA-48 and blaNDM-1 genes among carbapenem-resistant Enterobacteriaceae isolated from allograft liver recipients". EKB Journal Management System, 29, 3, 2020, 29-36. doi: https://doi.org/10.51429/EJMM29304
Awad, S., Ghoneim, E., Abd El Aziz, A., El Shaaraway, Y., Taha, M. (2020). 'Phenotypic tests and Molecular detection of blaOXA-48 and blaNDM-1 genes among carbapenem-resistant Enterobacteriaceae isolated from allograft liver recipients', EKB Journal Management System, 29(3), pp. 29-36. doi: https://doi.org/10.51429/EJMM29304
Awad, S., Ghoneim, E., Abd El Aziz, A., El Shaaraway, Y., Taha, M. Phenotypic tests and Molecular detection of blaOXA-48 and blaNDM-1 genes among carbapenem-resistant Enterobacteriaceae isolated from allograft liver recipients. EKB Journal Management System, 2020; 29(3): 29-36. doi: https://doi.org/10.51429/EJMM29304

Phenotypic tests and Molecular detection of blaOXA-48 and blaNDM-1 genes among carbapenem-resistant Enterobacteriaceae isolated from allograft liver recipients

Egyptian Journal of Medical Microbiology
Volume 29, Issue 3, July 2020, Page 29-36  XML PDF (521.06 K)
Document Type: New and original researches in the field of Microbiology.
DOI: https://doi.org/10.51429/EJMM29304
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Authors
Samah M. Awad* 1; Enas M. Ghoneim1; Azza M. Abd El Aziz1; Yasmin A. El Shaaraway1; Mohammad Taha2
1Department of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University, Egypt
2Depatment of Hepatopancreatobiliary surgery, National Liver Institute, Menoufia University, Egypt
Abstract
Background: Infections with carbapenem resistant Enterobacteriaceae (CRE) cause significant morbidity and mortality in Liver transplant recipients. Accurate detection of CRE is the first step in combating this problem. Objectives: to study the distribution of pathogens and CRE causing infections in liver allograft recipients with special reference to the impact of pre-operative intestinal colonization with CRE on post-operative infections, and to investigate the phenotypic tests and to detect the frequency of blaOXA-48 and blaNDM-1 genes by duplex PCR. Methodology: A total of 23 CRE isolates were investigated for carbapenemase production by phenotypic methods such as the modified Hodge test (MHT), douple disc synergy test (DDST), E test and Rabidec Carba NP (RCNP) test. Production of blaOXA 48 and blaNDM-1genes was investigated by duplex PCR. Results: Frequency of CRE that harbored blaOXA-48 and blaNDM-1 genes, as obtained by PCR, was 47.8% for blaOXA-48, 20.1% for blaNDM-1 and 8.7% of CRE isolates co-produced the two genes. RCNP test exhibited Sensitivity of 90.9% and 100% versus 81.1% and 66.6% sensitivities by MHT in detecting blaOXA-48 and blaNDM-1genes respectively. E-test had a much higher sensitivity in identifying NDM-1 carpabenemase compared to DDST (100% versus 66.6% respectively).Conclusion: RCNP test can be done instead of MHT in detecting CRE, as it is more accurate, rapid and having excellent sensitivity in detecting blaNDM-1 and blaOXA-48 producing isolates. E-test is more reliable than DDST and can be a valid alternative to PCR in detecting MBL especially NDM-1 type.
Keywords
CRE; Enterobacteraece; allograft liver recipient
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