THE DIAGNOSTIC ROLE OF CHOLINE TRANSPORTER AND CALRETININ IMMUNOHISTOCHEMISTRY IN HIRSCHSPRUNG DISEASE: A COMPARATIVE STUDY | ||||
| ALEXMED ePosters | ||||
| Article 3, Volume 4, Issue 3, September 2022, Page 16-17 | ||||
| Document Type: Preliminary preprint short reports of original research | ||||
| DOI: 10.21608/alexpo.2022.152328.1442 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Layla Kamal Younis1; Dina Abdalla2; Mohammed Abd AlAziz Al Sherif3; Mohamed Mohamed Aboraya  1
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| 1Department of Pathology, Faculty of Medicine, Alexandria University. | ||||
| 2pathology department, faculty of medicine, Alexandria university, Egypt | ||||
| 3Department of Surgery, Armed Forces Medical Academy | ||||
| Abstract | ||||
| Hirschsprung’s disease is an ENS malformation, with absence of intrinsic gangliain the distal rectum and a variable length of proximal bowel. Microscopic picture in H&E stained slides include absence of ganglion cells in muscularispropria and submucosa, and hyperplasia of cholinergic nerves & neurites. Diagnosis is challenging as, it needs hard boring examination of numerous sections of the tiny suction biopsy which lake the deep submucosa & muscularispropria where the ganglion cells are abundant, searching for ganglion cells which has a normal sparse nature in the distal 1-2 cm of the rectum. So we need ancillary techniques to help in diagnosis and make it easier, previously it was used AChE EHC which needs fresh frozen sample & CR IHC. Here we study Choline Transporter IHC to be a surrogate for AChE EHC with the advantage of being used with FFPE tissue sections, and to help in cases with misleading calretinin results. | ||||
| Keywords | ||||
| HIRSCHSPRUNG; CALRETININ; CHOLINE TRANSPORTER | ||||
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