THE DIAGNOSTIC ROLE OF CHOLINE TRANSPORTER AND CALRETININ IMMUNOHISTOCHEMISTRY IN HIRSCHSPRUNG DISEASE: A COMPARATIVE STUDY | ||
| ALEXMED ePosters | ||
| Article 3, Volume 4, Issue 3, September 2022, Pages 16-17 | ||
| Document Type: Preliminary preprint short reports of original research | ||
| DOI: 10.21608/alexpo.2022.152328.1442 | ||
| Authors | ||
| Layla Kamal Younis1; Dina Abdalla2; Mohammed Abd AlAziz Al Sherif3; Mohamed Mohamed Aboraya* 1 | ||
| 1Department of Pathology, Faculty of Medicine, Alexandria University. | ||
| 2pathology department, faculty of medicine, Alexandria university, Egypt | ||
| 3Department of Surgery, Armed Forces Medical Academy | ||
| Abstract | ||
| Hirschsprung’s disease is an ENS malformation, with absence of intrinsic gangliain the distal rectum and a variable length of proximal bowel. Microscopic picture in H&E stained slides include absence of ganglion cells in muscularispropria and submucosa, and hyperplasia of cholinergic nerves & neurites. Diagnosis is challenging as, it needs hard boring examination of numerous sections of the tiny suction biopsy which lake the deep submucosa & muscularispropria where the ganglion cells are abundant, searching for ganglion cells which has a normal sparse nature in the distal 1-2 cm of the rectum. So we need ancillary techniques to help in diagnosis and make it easier, previously it was used AChE EHC which needs fresh frozen sample & CR IHC. Here we study Choline Transporter IHC to be a surrogate for AChE EHC with the advantage of being used with FFPE tissue sections, and to help in cases with misleading calretinin results. | ||
| Keywords | ||
| HIRSCHSPRUNG; CALRETININ; CHOLINE TRANSPORTER | ||
|
Supplementary Files
|
||
|
Statistics Article View: 263 |
||
