MICROPROPAGATION OF Eucalyptus grandis TREE,S | ||||
| Journal of Plant Production | ||||
| Article 16, Volume 28, Issue 7, July 2003, Page 5557-5566 PDF (1.32 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2003.252515 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| S. L. Maxlmous,; M. M. E. Abd EI-Kader | ||||
| Hort. Res. Inst, Forestry Res. Dept., Agric., Res. Center., Giza Egypt. | ||||
| Abstract | ||||
| This work aimed to study the effect of medium type, explant part and kinitin concentration on in vitro multiplication as well as in vitro rooting of Eucalyptus grandis to obtain the optimum regeneration phase and to develop the propagation method of E, grandis The results indicated that, the highest number of formed shoots was regarded with culturing the basal node on MS medium supplemented with 2mg/litre kin. as compared with WPM medium. All treatments (is MS medium supplemented with ISA) induced root formation on E. grandis shoots, but ISA at 2mgllitre recorded the best results. The rooted shootlets (plantlets) were removed from rooting medium after seven weeks and cultured in plastic pots 10 cm. diameter, containing peatmoos, clay and sand (1:1:1 by volume) under greenhouse conditions for four weeks. The survival percentage of E. gandis plants recorded 83% . from the above results. the micropropagation techniques strongly recommended to increase the number of E. grandis plants in Egypt. | ||||
| Keywords | ||||
| Micropropagation; invitro; tissue culture; explants; ISA; Kinetin; media type; MS; WPM; rooling .shooting; media sirength; multiplication. Eucalyptus grandis | ||||
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