STUDIES ON THE BIOSYNTHESIS OF L- ALANINE DEHYDROGENASE BY Thielaviopsis paradoxa | ||||
| Journal of Plant Production | ||||
| Article 14, Volume 27, Issue 3, March 2002, Page 1591-1600 PDF (749.13 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2002.253503 | ||||
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| Author | ||||
| Siham A. Al- Kadeeb, | ||||
| Botany Department ,Girls College of Education in Riyadh , Kingdom of Saudia Arabia | ||||
| Abstract | ||||
| Cell free extracts of Thielaviopsis paradoxa grown on L- alanine as the sole source of nitrogen contained L- alanine dehydrogenase that catalyzes the oxidative deamination of L- alanine to equimolar amounts of pyruvate and ammonia . L- alanine dehydrogenase formation takes place during the logarithmic phase of growth .Maximal growth and enzyme formation were obtained at the 4th day of growth .The optimum pH for growth and enzyme synthesis was 4.0 . L- alanine dehydrogenase was induced by a great variety of nitrogen sources , but L –alanine , DL- alanine, L-theronine and ammonium phosphate were the most potent inducers . L-alanine concentration of 2.1 g/ l was found to be optimum for both growth and enzyme synthesis . L- alanine dehydrogenase formation was increased with increasing concentrations of ammonium phosphate in the growth medium ; the optimum concentration was 2.6 g/ l . The effect of different carbon sources and metal salts on growth and enzyme production was studied . | ||||
| Keywords | ||||
| Thielaviopsis paradoxa; Lـ alanine dehydrogenase synthesis | ||||
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