Role of SARS-COV2 Antigen as a diagnostic test for COVID-19 | ||||
| Microbes and Infectious Diseases | ||||
| Article 7, Volume 3, Issue 4, November 2022, Page 808-813 PDF (323.64 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2022.150013.1345 | ||||
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| Authors | ||||
Raafat Z. Abd el rahman1; Ahmed G. Taha1; Heba Fawzy1; Mahmoud K. Hussein1; fatma M Mahmoud   2
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| 1Medical Virology Department, The Armed Central Laboratories for Medical Research and Blood Bank, Cairo, Egypt | ||||
| 2Medical Microbiology and Immunology department, Faculty of medicine, Ain Shams University, Cairo, Egypt | ||||
| Abstract | ||||
| Background: Successive waves of SARS-CoV-2 infections with increasing transmission rates may burden the laboratories performing molecular diagnostic testing. Alternative diagnostic methods may provide additional diagnostic capacity. Chemiluminescent totally automated antigen detection test for SARS-CoV-2 (Ortho VITROS SARS-CoV-2 antigen test) could be satisfactory replacement for reverse-transcription quantitative polymerase chain reaction (RT-qPCR) for mass screening during outbreaks. Methods: RT- qPCR and the VITROS® SARS-CoV-2 antigen were compared. Antigen detection test was assessed using clinical samples (nasopharyngeal swabs in viral transport medium) withdrawn from 668 patients suspected to have SARS-CoV-2 infection. Results: From 668 samples, 303 showed SARS-CoV-2 antigens positive and 365 SARS-CoV-2 antigens negative in comparison with RT-qPCR, the sensitivity was 89.11% and the specificity was 100.0% (PPV 100.0 and NPV 91.7). Ct value of 16.0 was the limit of detection of the assay. Conclusion: The given results show that VITROS® assay was acceptable for the detection of patients having contagious COVID-19 in the clinical setting. This test showed high sensitivity and specificity in the SARS-CoV-2 detection in samples with a Ct value of 32 or less. Chemiluminescent full automated antigen detection test for SARS-CoV-2 is a feasible substitute to (RT-qPCR) for mass screening | ||||
| Keywords | ||||
| SARS-COV-2; SARS-CoV-2 antigen; RT-qPCR | ||||
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