Prevalence, phenotypic characterization of listeria monocytogenes isolated from diseased sheep

Ezzat, Mahmoud; ibrahim, marwa; ELgedway, Attia; ELTarabili, Reham Mokhtar; arnout, marwa

doi:10.21608/scvmj.2022.145088.1087

	Prevalence, phenotypic characterization of listeria monocytogenes isolated from diseased sheep
Suez Canal Veterinary Medical Journal. SCVMJ
Articles in Press, Accepted Manuscript, Available Online from 10 August 2022
Document Type: Original Article
DOI: 10.21608/scvmj.2022.145088.1087
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Authors
Mahmoud Ezzat ¹; marwa ibrahim²; Attia ELgedway³; Reham Mokhtar ELTarabili ⁴; marwa arnout⁵
¹Professor of Microbiology, Faculty of Veterinary Medicine, Suez Canal University
²microbiology department,faculty of veterinary medicine,Zagazig university
³Microbiology Department, Animal Health Research Institute ,Dokki ,Egypt
⁴bacteriology,immunoloy and mycology department, faculty of veterinary medicine , Suez Canal University, Ismailia, Egypt
⁵veterinary quarintine,Cairo airport
Abstract
Listeriosis is the third most common foodborne illness and it is considered one of the most dangerous bacterial zoonotic infections globally. This research aimed to isolate and characterize Listeria monocytogenes (L. monocytogenes) from diseased sheep in Egypt. A total of 240 samples collected aseptically from sheep were subjected to isolation and biochemical characterization of L. monocytogenes. L. monocytogenes, which are Gram-positive rods that are both aerobic and facultatively anaerobic. Thirty-one listeria isolates (12.9%) were recovered from diseased sheep. All tested isolates were positive for catalase, Voges-Proskauer, carbohydrate fermentation, esculin, gelatin hydrolysis and methyl red tests. Meanwhile, they were negative for oxidase, indole, urease and nitrate reduction tests and they did not utilize citrate in the citrate utilization test. Listeria isolates produced yellow butt/yellow slant on TSI agar medium with no H2S or gas production. Therefore, this work focused on isolation of L. monocytgenes from diseased sheep and biochemical characterization of the recovered isolates using traditional and Microbact™ 12L listeria identification system.
Keywords
Listeria monocytogenes; sheep; phenotypic identification; biochemical tests


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