EFFECT OF APPLICATION TIME OF THREE ISOLATES OF Trichoderma harzianum ON THE BIOCONTROL OF SORGHUM DAMPING-OFF | ||||
| Journal of Plant Production | ||||
| Article 13, Volume 27, Issue 4, April 2002, Page 2155-2171 PDF (4.39 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2002.253972 | ||||
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| Authors | ||||
| Shahda T. T. Waffa,; Amal A. H. Hemeda; M. R. A. Shehata | ||||
| Plant Pathology Department, Faculty of Agriculture, El-Shatby, Alexandria University, Egypt. | ||||
| Abstract | ||||
| Three isolates of Trichoderma harzianum i.e. Isolate 1, (354.33, Netherlands), Isolate 2 (63059, Germany) and Isolate 3 (64256, Germany) were applied to the soil at different times for the control of Drechslera sp < /em>., three isolates of Fusarium moniliforme and Phoma sp < /em>., the causal organisms of sorghum damping-off under greenhouse conditions. The biocontrol fungi were applied to the soil either before, at the time or after sowing. The addition of Trichoderma isolates one week after sowing proved to be the most effective in reducing the disease except in case of Phoma sp < /em>. whereas the isolates application one week before sowing was better for disease control up to 8 weeks of sowing. Trichoderma 12 was the most effective if added before sowing as it reduced mortality percentage from 65.9% to 39.6%. However 11 was better at the time of sowing as it reduced the disease from 48.8% to 40.4%. Trichoderma 13 was the most effective when applied after sowing as it reduced mortality percentage from 50% to 36.3%. Maximum shoot length was achieved by using Trichoderma 11 before (72.7 cm) or at the time of sowing (51.8 cm) or [2 after sowing (88.8 cm). Maximum root length was achieved by using Trichoderma 13 before (31.4 cm) or after sowing (21.8 ern) and 11 at the. time of sowing (27.4 em), Trichoderma 11 gave the highest dry weight of shoot (before and at the time of sowing) and root (after and at the time of sowing), however, Trichoderma 12 gave the maximum dry weight of shoot (after sowing) and root (before sowing). These results indicated that although the use of the three isolates decreased the disease from 54.9% to 44.6%, but the effectiveness of the isolates of Trichoderma harzianum in controlling the disease and promoting growth depends on the used isolate and the time of application. | ||||
| Keywords | ||||
| T. harzianum; sorghum; damping-off; F. moniliforme; Drechslera sp; Phoma sp | ||||
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