In vitro CULTURE AND ESTABLISHMENT OF Dianthus caryophyl/us L. | ||||
| Journal of Plant Production | ||||
| Article 25, Volume 27, Issue 4, April 2002, Page 2459-2473 PDF (4.63 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2002.254001 | ||||
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| Authors | ||||
| Eman l. Maghazy; H. K. Atta-Alla; A. K. Waly; I. H. Abo El Soad | ||||
| Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia 41522, Egypt. | ||||
| Abstract | ||||
| Shoot tips of Dianthus caryophyl/us L. cvs., Lelia, Maragia, Sorriso and Aicardi were excised from the plants grown in the greenhouse and cultured on MS medium containing SA at 0.0, 0.5, 1.0 and 2.0 mgll. The medium was solidified with 8.0, 10.0, 12.0 and 14.0 gIl agar. After 8 weeks, the results showed that the treatment with SA at 2.0 mgll and agar at 8.0 gIl produced the highest number of shoots as 8.3 and 10.1 shoots lexplant with Maragia and Aicardi cultivars. Increasing SA levels in the culture medium increased vitrification percentage. On the other side, increasing agar concentration decreased both the multiplication rate and the percentage of vitrified shoots. The developed shoots were transferred to. a rooting medium (MS medium supplemented with NAA at 0.0 and 1.0 mg/l).The medium was solidified with agar at 7.0 or 8.0 gIl. The treatment with MS medium containing NAA at 1.0 mg/l produced the largest number of roots with Maragia and Sorriso cultivars. The plantlets were then transferred from the rooting medium and cultured on small plastic pots containing peatmoss: vermiculite and perlite. (1: 1 :1. vv:v). The pots were kept under mist system for 2 weeks. The plants grew normally and the survival percentage reached 80% with all cultivars. Abbreviations: SA- Senzyladenine; MS- Murashige and Skoog (1962) medium; NAA- Naphathaleneacetic acid | ||||
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