Maceration of some plant pectic materials with aspergillus niger pectinases produced on orange peel with solid state fermentation | ||||
Journal of Agricultural Chemistry and Biotechnology | ||||
Article 1, Volume 27, Issue 4, April 2002, Page 2527-2543 PDF (4.73 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jacb.2002.254154 | ||||
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Authors | ||||
T. S. M. Shady; W. I. A. Saber; Nadia A. A. Ali; Amal M. El-Bastawesy | ||||
Abstract | ||||
Different forms' C{'pectinase's liffi>'procfucel:r by the fungal isolates tested. Aspergilius niger No. 30 was found as the most active strains tested for PG, PGL, PMG, PMGL and PMGE biosynthesis, Asp < /em>ergillus achuleatus was highest pectinases !61~~rs, but found in the second order. Enzymes productivity reached its maximum afte(i~120, 96, 144, 120 and 144 hours of incubation for PG, PGL, PMG, PMGL and iJ l~~ir respectively. The highest biosynthesis of pectinases were observed at 30°C ~ri1'~~t1.0 in the medium containing 3% 0NN) sugar cane molasse as carbon and enE?J.9Y source. 0.07% and 0.105% as nitrogen content of corn steep liquor induced greatly' PG, PMG, PMGE and PGL, PMGLase activities, respectively. A. niger cultures produced highest amount of pectinases between 0.5: 1 to 0.7: 1 (solid waste to liquid ratio of the fermentation media. PH 4, 7, 6, 8 and 6 were round as the optimum pH for PG, PGL, PMG, PMGL and PMGE, respectively. A. niger pectinases were found stable between pH 4.0 to pH 8.0. 30, 40, 50, 50 and 40°C were found as the optimum temperature for these enzymes, respectively. These enzymes were also highest stable up to 60°C, then decreased gradually. Some of meta! ions such as Na+ induced greatly pectinases activity, but other such as Hg+2 reduced greatly these enzymes activities. Aspergillus niger pectinases system success with higher grade to hydrolysis some plant materials, this is due to its having pectate and pectin enzymes. Therefore, these enzymes systems well useful in the biotechnological process of fruit and vegetable tissues such as fruit and vegetable extraction and clarification. | ||||
Keywords | ||||
Fungi; Aspergillus niger; pectinase; PG; PGL; PMG; PMGL; PMGE; solid- state fermentation; maceration | ||||
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