DEGRADATION OF PLANT PATHOGENIC FUNGI BY Streptomycetes | ||||
Journal of Soil Sciences and Agricultural Engineering | ||||
Article 9, Volume 27, Issue 4, April 2002, Page 2761-2774 PDF (3.96 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jssae.2002.254167 | ||||
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Author | ||||
Moataza M. Saad, | ||||
Microbial Chemistry Department National Research Center, Dokki, Egypt | ||||
Abstract | ||||
An isolate of Strep < /em>tomyces sp. excreted chitinase, cellulase and protease activities into the medium when grown on chitin, carboxymethylcellulose and casein ,respectively, or on cell walls of the fungal pathogen Sclerotium rolfsii or Rhizoctonia solni, as sole carbon source. This isolate of Streptomyces sp. gave highest lytic enzymes ( chitinase , cellulase, protease ), when grown at28 °C. Factors involved in the production of enzymes were investigated. The lytic activities were produced optimally in the medium at' pH range between 6.5-7.0 for the three enzymes . The lytic activities of culture on autoclaved mycelium of S. rolfsii and R .. solani took place at different pH values .The effect of mycelium concentration as substrate on the extent on mycelium lysis and lytic activities were also studied. The level of hydrolytic enzymes, produced by Streptomyces sp. depended on the source of mycelium attacked i. e. S. rolfsii or R. so/ani in soil. | ||||
Keywords | ||||
Streptomyces sp; Biological control; Lytic enzyme; Wilt disease | ||||
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