Sensitive spectrofluorimetric determination of metoclopramide, itopride, mosapride, and trimebutine by Fluorescence Quenching Reaction with Eosin Y in Pharmaceutical Formulations and Spiked Human Plasma.
Saraya, R., salman, B. (2022). Sensitive spectrofluorimetric determination of metoclopramide, itopride, mosapride, and trimebutine by Fluorescence Quenching Reaction with Eosin Y in Pharmaceutical Formulations and Spiked Human Plasma.. EKB Journal Management System, 1(1), 10-24. doi: 10.21608/odr.2022.154538.1004
Roshdy elsayed Saraya; baher ibrahim salman. "Sensitive spectrofluorimetric determination of metoclopramide, itopride, mosapride, and trimebutine by Fluorescence Quenching Reaction with Eosin Y in Pharmaceutical Formulations and Spiked Human Plasma.". EKB Journal Management System, 1, 1, 2022, 10-24. doi: 10.21608/odr.2022.154538.1004
Saraya, R., salman, B. (2022). 'Sensitive spectrofluorimetric determination of metoclopramide, itopride, mosapride, and trimebutine by Fluorescence Quenching Reaction with Eosin Y in Pharmaceutical Formulations and Spiked Human Plasma.', EKB Journal Management System, 1(1), pp. 10-24. doi: 10.21608/odr.2022.154538.1004
Saraya, R., salman, B. Sensitive spectrofluorimetric determination of metoclopramide, itopride, mosapride, and trimebutine by Fluorescence Quenching Reaction with Eosin Y in Pharmaceutical Formulations and Spiked Human Plasma.. EKB Journal Management System, 2022; 1(1): 10-24. doi: 10.21608/odr.2022.154538.1004

Sensitive spectrofluorimetric determination of metoclopramide, itopride, mosapride, and trimebutine by Fluorescence Quenching Reaction with Eosin Y in Pharmaceutical Formulations and Spiked Human Plasma.

Octahedron Drug Research
Article 2, Volume 1, Issue 1, September 2022, Page 10-24  XML PDF (661.88 K)
Document Type: Research Articles
DOI: 10.21608/odr.2022.154538.1004
View on SCiNiTO View on SCiNiTO
Authors
Roshdy elsayed Saraya email orcid 1; baher ibrahim salmanorcid 2
1Analytical Chemistry Department, Faculty of Pharmacy, PortSaid University
2al azhar university faculty of pharmacy assiut branch
Abstract
In order to determine the levels of metoclopramide hydrochloride (MTC), itopride hydrochloride (ITP), mosapride citrate (MR), and trimebutine maleate (TRI) in their pharmaceutical formulations and spiked human plasma, a new straightforward, accurate, highly sensitive, non-extractable, economical, and quick spectrofluorimetric method was developed. The technique was based on the reaction between the drug's tertiary amino group and eosin Y (EY), which causes quantitative fluorescence quenching of EY, which was detected at 542 nm after excitation at 520 nm. For MTC, ITP, and MS, the quenching in fluorescence intensity was directly proportional to the drug concentrations, while for TRI, it was between 100 and 1000 ng ml-1. And with a quantitation limit of 24, 40, 4, and 25 ng ml-1 for MTC, ITP, MR, and TRI, as well as detection limits of 8 ng ml-1, 13.5 ng ml-1, 12.1 ng ml-1, and 8.77 ng ml-1. According to ICH requirements, the proposed analytical method was validated, and the outcomes were satisfactory. The researched medicines' presence in pharmaceutical formulations and spiked human plasma has been successfully determined using the suggested analytical method, which also provides an excellent percent recovery. The outcomes are quite good. agreement with the reported method with respect to precision and accuracy
Keywords
Metoclopramide; itopride; Mosapride; trimebutine; Eosin Y
Statistics
Article View: 315
PDF Download: 462