Prevalence of Enterococci and Streptococci in Raw Milk and Some Dairy Products and The Subsequent Alteration on Quality
Gorgy, S., S., E., S., S., Ali, A. (2016). Prevalence of Enterococci and Streptococci in Raw Milk and Some Dairy Products and The Subsequent Alteration on Quality. EKB Journal Management System, 2(2), 500-515. doi: 10.21608/ejceh.2016.254610
Seham F. Gorgy; El Asuoty M. S.; Saber A. S.; Abeer H. Ali. "Prevalence of Enterococci and Streptococci in Raw Milk and Some Dairy Products and The Subsequent Alteration on Quality". EKB Journal Management System, 2, 2, 2016, 500-515. doi: 10.21608/ejceh.2016.254610
Gorgy, S., S., E., S., S., Ali, A. (2016). 'Prevalence of Enterococci and Streptococci in Raw Milk and Some Dairy Products and The Subsequent Alteration on Quality', EKB Journal Management System, 2(2), pp. 500-515. doi: 10.21608/ejceh.2016.254610
Gorgy, S., S., E., S., S., Ali, A. Prevalence of Enterococci and Streptococci in Raw Milk and Some Dairy Products and The Subsequent Alteration on Quality. EKB Journal Management System, 2016; 2(2): 500-515. doi: 10.21608/ejceh.2016.254610

Prevalence of Enterococci and Streptococci in Raw Milk and Some Dairy Products and The Subsequent Alteration on Quality

Egyptian Journal of Chemistry and Environmental Health
Volume 2, Issue 2, November 2016, Page 500-515  XML PDF (386.22 K)
Document Type: Scientific and Research
DOI: 10.21608/ejceh.2016.254610
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Authors
Seham F. Gorgy* 1; El Asuoty M. S.2; Saber A. S.2; Abeer H. Ali3
1Animal Health Research Institute
2Animal Health Research Institute, Damanhour branch
3Alexandria branch AHRI, Egypt
Abstract
The purpose of this research was to study the prevalence of enterococci and streptococci in milk and some dairy products. Survey included 50 samples of raw milk, 25 kareish cheese and 25 yoghurt samples which were collected from El-Behera governorate markets. The results revealed that the mean of chemical composition was 12.95±0.11, 86.34±0.26, 6.3±0.07 and 0.18±0.002 for total solid, Moisture content, pH values and titratable acidity, respectively. in raw milk samples; 36.8±0.87, 61.76±0.84, 3.87±0.11 and 2.27±0.037 in kareish cheese and 36.8±0.87, 76.48±0.94, 4.33±0.05 and 1.09±0.036 in yoghurt samples, Respectively. incidence of positive samples for aerobic plate count, streptococci and enterococci count were 88 , 26 and 22 % in raw milk samples, respectively . 76 , 36 and 36 % in kareish cheese and 56, 28 and 32 % in yoghurt samples, respectively. with mean values of 1.22x105 ±0.1x105, 4.5x103 ±0.7x103 and 6.3x103 ±1.9x103 cfu/ml in raw milk samples; 8.9x104 ±0.76x104 , 4.7x103 ±0.87x103  and 5.7x103 ±1.6x103 cfu/gm in kareish cheese samples and in yoghurt samples were 7.6x104 ±0.59x104 , 3.7x103 ±0.6x103 and 5.5x103 ±0.64x103 , respectively. All isolated Enterococcus faecium, Enterococcus faecalis, S. agalactiae and S. dysgalatiae were confirmed at a genus level using specific primer targeting 16s rRNA gene in S. dysgalatiae and Enter. Faecalis; sod A gene in enterococcus faecium and cfb gene in S. agalactiae. Desired amplicon for virulence genes were obtained.
Keywords
milk; dairy products; enterococci; streptococci; PCR; 16s rDNA
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