USE OF MORPHOLOGICAL TRAITS AND ELECTRO- PHORESIS OF PROTEINS TO IDENTIFY THE TELEOMORPH OF Oidium lini INVOLVED IN POWDERY MILDEW OF FLAX | ||||
Journal of Plant Production | ||||
Article 9, Volume 27, Issue 6, June 2002, Page 3755-3765 PDF (2.82 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jpp.2002.254901 | ||||
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Authors | ||||
A. A. Aly,1; E. M. Hussein1; Nawal A. Eisa2; R. N. Fawzy2; M. T. M. Mansour1 | ||||
1Plant Pathology Research Institute, Agricultural, Res. Center, Giza, Egypt. | ||||
2Dept. of Agric. Botany, Faculty of Agric., Zagazig Univ., Moshtohor, Egypt. | ||||
Abstract | ||||
Powdery mildew (PM) of flax is caused by the obligate parasite Odium lini. One obvious gap in our current knowledge of O. lini is the lack of consensus regarding the name of its teleomorph, which has been referred to as Erysiphe polygoni or E. cichoracearum. As an attempt to reslove this problem, this study used biostatistical analysis (BSA) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) of proteins to evaluate the degree of relatedness between O. lini and some anamorphs of PM fungi, whose teleomorphic taxonomy is well established. In BSA, measurements were made for some quantitative morphological traits of the anamorphs of various fungi belong to E. cichoracearum and E. polygoni. The obtained measurements were subjected to cluster analysis by the unweighted pair-group method based on arithmetic means (UPGMA) and a phenogram was constructed. This phenogram showed that morphotypes 1 and 2 of O. lini belong to E. polygoni, while morphotype 3 belongs to E. cichoracearum. In SDS-PAGE, protein profiles of O. lini were compared with those of E. polygoni from peas and E. cichoracearum from sunflower. On the basis of electrophoretic dissimilarities among protein banding patterns, fungi were grouped by cluster analysis (UPGMA) and the results were expressed as a phenogram. This phenogram confirmed that morphotypes 1 and 2 of O. lini belong to E. polygoni. | ||||
Keywords | ||||
Oidium lini; Morphology; SDS-PAGE | ||||
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