THE USE OF LOW QUALITY SWEET POTATOES ROOTS (Ipomoea batatas) FOR ALPHA·AMYLASE PRODUCTION BY Aspergillus oryzae | ||||
| Journal of Soil Sciences and Agricultural Engineering | ||||
| Article 1, Volume 27, Issue 7, July 2002, Page 5003-5012 PDF (995.41 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jssae.2002.255179 | ||||
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| Author | ||||
| A.E.I. Salim | ||||
| Dept. of Agrlc. Mlcroblol., Fac. of Agrlc., Mansoura Un Iv., Egypt. | ||||
| Abstract | ||||
| Low quality sweet potatoes roots were used as a carbon source to produce extracellular alpha-amylase by Asperigiffus arrzae in shake flask cultures. Peak alpha - amylase production was obtained by local strain of Aspergillus oryzae on sweet potatoes filtrate medium amended with saHs solutions and sodium nitrate, pH 6.0 after incubation at 3Q·C for 5 days. The optimum temperature and pH of the enzyme activity was 50"C and 5.6, respectively. The optimum time for enzyme actiVity, using starch buffered 0.5%, was 1h at optimum pH 5.6 and optimum temperature 50·C. The produced enzyme is stable at temperatures below 50·C, while the enzyme lost 13.1, 24.5,46.8 and 57.2% of its original activity after exposure at 55, 60,65 and 75·C for 1h., respectively. | ||||
| Keywords | ||||
| alpha; amylase; Low quality sweet potatoes; Aspergillus arrzae | ||||
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