Exposure to copper nanoparticles induces oxidative stress and alters Hsp70 and Sod2 gene expression in Drosophila melanogaster
Saudi, M., Said, O., Abdel-Samie, E., Abdel-Hakim, M. (2022). Exposure to copper nanoparticles induces oxidative stress and alters Hsp70 and Sod2 gene expression in Drosophila melanogaster. EKB Journal Management System, 60(2), 36-50. doi: 10.21608/ejaps.2022.135803.1033
Maha Saudi; Osama Said; Emtithal Abdel-Samie; Mohamed Abdel-Hakim. "Exposure to copper nanoparticles induces oxidative stress and alters Hsp70 and Sod2 gene expression in Drosophila melanogaster". EKB Journal Management System, 60, 2, 2022, 36-50. doi: 10.21608/ejaps.2022.135803.1033
Saudi, M., Said, O., Abdel-Samie, E., Abdel-Hakim, M. (2022). 'Exposure to copper nanoparticles induces oxidative stress and alters Hsp70 and Sod2 gene expression in Drosophila melanogaster', EKB Journal Management System, 60(2), pp. 36-50. doi: 10.21608/ejaps.2022.135803.1033
Saudi, M., Said, O., Abdel-Samie, E., Abdel-Hakim, M. Exposure to copper nanoparticles induces oxidative stress and alters Hsp70 and Sod2 gene expression in Drosophila melanogaster. EKB Journal Management System, 2022; 60(2): 36-50. doi: 10.21608/ejaps.2022.135803.1033

Exposure to copper nanoparticles induces oxidative stress and alters Hsp70 and Sod2 gene expression in Drosophila melanogaster

Egyptian Journal of Pure and Applied Science
Article 4, Volume 60, Issue 2, June 2022, Page 36-50  XML PDF (798.74 K)
Document Type: Original Article
DOI: 10.21608/ejaps.2022.135803.1033
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Authors
Maha Saudi1; Osama Said2; Emtithal Abdel-Samie3; Mohamed Abdel-Hakim email 1
1Department of Pharmaceutical Biotechnology, College of Biotechnology, Misr University for Science and Technology, Giza, Egypt
2Department of Agriculture Biotechnology, College of Biotechnology, Misr University for Science and Technology, Giza, Egypt.
3Entomology Department, Faculty of Science, Cairo University, Giza, Egypt.
Abstract
Copper nanoparticles (CuNPs) are used in a range of industries such as semiconductors, catalysts, sensors, and antimicrobial agents. While there are already studies on its possible genotoxicity, few of these reports evidence in vivo. Copper nanoparticles (CuNPs) were prepared via chemical reduction and characterized by electronic transmission microscopy (TEM) and X-ray diffraction. Drosophila melanogaster (D. melanogaster) were reared on CuNPs, and Cu+2 (as CuSO4) treated food from egg to egg stage. The total number of progeny, percentage of aberrant phenotypes, oxidative stress, and gene expression of Hsp70 and Sod2 were investigated.
Results showed that the acute exposure of CuNPs did not affect the fly's survivorship, unlike Cu+2, which showed higher toxicity. Chronic exposure of D. melanogaster to CuNPs (100 ppm) and Cu+2 (50 and 100 ppm) resulted in a delay in the development of three consecutive generations. Furthermore, the ingestion of Cu+2 and CuNPs during early developmental stages caused a dose-dependent reduction in the number of emerged flies. CuNPs and Cu+2 treatments resulted in distinctive phenotypic aberrations, such as deformed wings transmitted to the offspring in subsequent generations. Finally, CuNPs and Cu+2 treatments caused downregulation of the Sod2 gene and upregulation of the Hsp70 gene in the second and third generations. This study indicated that CuNPs are mutagenic for D. melanogaster. So, it is necessary to evaluate CuNPs toxicity to reduce human health-related issues.
Keywords
Drosophila; copper nanoparticles; oxidative stress; nanotoxicology
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