Hexavalent Chromium Reduction by a Potent Novel Haloalkaliphilic Nesterenkonia sp strain NRC-Y Isolated from Hypersaline Soda Lakes
Abo Alkasem, M., El-Abd, M., Maany, D., Ibrahim, A. (2023). Hexavalent Chromium Reduction by a Potent Novel Haloalkaliphilic Nesterenkonia sp strain NRC-Y Isolated from Hypersaline Soda Lakes. EKB Journal Management System, 66(6), 463-473. doi: 10.21608/ejchem.2022.136986.6039
Mohamed Ibrahim Abo Alkasem; Mostafa Abd El-Hameed El-Abd; Dina Abdelfattah Maany; Abdelnasser Salah Shebl Ibrahim. "Hexavalent Chromium Reduction by a Potent Novel Haloalkaliphilic Nesterenkonia sp strain NRC-Y Isolated from Hypersaline Soda Lakes". EKB Journal Management System, 66, 6, 2023, 463-473. doi: 10.21608/ejchem.2022.136986.6039
Abo Alkasem, M., El-Abd, M., Maany, D., Ibrahim, A. (2023). 'Hexavalent Chromium Reduction by a Potent Novel Haloalkaliphilic Nesterenkonia sp strain NRC-Y Isolated from Hypersaline Soda Lakes', EKB Journal Management System, 66(6), pp. 463-473. doi: 10.21608/ejchem.2022.136986.6039
Abo Alkasem, M., El-Abd, M., Maany, D., Ibrahim, A. Hexavalent Chromium Reduction by a Potent Novel Haloalkaliphilic Nesterenkonia sp strain NRC-Y Isolated from Hypersaline Soda Lakes. EKB Journal Management System, 2023; 66(6): 463-473. doi: 10.21608/ejchem.2022.136986.6039

Hexavalent Chromium Reduction by a Potent Novel Haloalkaliphilic Nesterenkonia sp strain NRC-Y Isolated from Hypersaline Soda Lakes

Egyptian Journal of Chemistry
Volume 66, Issue 6, June 2023, Page 463-473  XML PDF (755.01 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.136986.6039
View on SCiNiTO View on SCiNiTO
Authors
Mohamed Ibrahim Abo Alkasemorcid 1; Mostafa Abd El-Hameed El-Abdorcid 1; Dina Abdelfattah Maanyorcid 1; Abdelnasser Salah Shebl Ibrahim email 2
1Department of Chemistry of Natural and Microbial Products, Pharmaceutical and Drug Industries Research Institute, National Research Centre, El-Buhouth St., Dokki 12622, Cairo, Egypt
2Department of chemisrty of natural and Microbial prodycts; Pharmaceutical and drug industries research institute; National Research Centre, Cairo; Egypt
Abstract
We reported a novel haloalkaliphilic bacterial strain NRC-Y with remarkable hexavalent chromium reduction efficiency and studied its detoxification mechanism. Strain NRC-Y was identified based on 16S-rDNA sequence analysis as Nesterenkonia sp. Strain NRC-Y exhibited high Cr6+ reduction efficiency under a wide range of sodium chloride concentration, pH, shaking velocity, and temperature, showing maximum Cr6+ reduction at 6% NaCl, pH 9, 150 rpm and 40ºC, respectively. In addition, strain NRC-Y could grow and reduce Cr6+ effectively in the presence of a variety of heavy metals and oxyanions. The co-existence of Cd2+ and Zn2+ caused a slight decrease in the Cr6+reduction, while Fe3+ and Cu2+ ions significantly increased the Cr6+ reduction by 1.7- and 1.8-fold, respectively. Among the tested electron donors, maximum Cr6+ reduction was observed using glycerol. Cell fractionation experiments proved that Cr6+ bioremoval occurred because of chromate enzyme activity, which was detected mainly in the bacterial cell membranes (73.2%) and cytoplasm (22.6%). SEM–EDX analyses confirmed the biosorption of chromium species into the cells. Strain NRC-Y exhibited a potent Cr6+ bioreduction efficiency, which could reduce up to 800 mgL−1 within 24 h. Therefore, strain NRC-Y is a promising candidate in the bioremediation of Cr6+ contaminated sites, particularly under harsh conditions. Further investigation using chromium containing industrial waste is recommended.
Keywords
Bioremediation; Extremophiles; Haloalkaliphiles; Hexavalent chromium
Statistics
Article View: 357
PDF Download: 170