Effects of Thawing Methods (Techniques) on Freeze Thaw Buffalo Bull Sperm Quality | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Volume 53, Issue 4, December 2022, Page 591-597 PDF (1.34 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2022.130902.1333 | ||||
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| Authors | ||||
Saima Qadeer  1; Asma Ul Husna2; Rajbari Rajbari3; Roma Adalat3; Asima Azam 4; rabea ejaz5; Shamim Akhtar6
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| 1Department of Zoology, Division of Science and Technology, University of Education Lahore, Jauharabad Campus | ||||
| 2Department of Biology, University of Haripur, 22620, Khyber Pakhtunkhwa- Pakistan | ||||
| 3Department of Zoology, University of Sialkot-51310, Punjab- Pakistan | ||||
| 4Department of Zoology, Shaheed Benazir Bhutto Women University, Peshawar | ||||
| 5Department of Zoology, Shaheed Benazir Bhutto Women University Peshawar, 25000, Pakistan | ||||
| 6Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi-46300, Pakistan | ||||
| Abstract | ||||
| Background: Semen cryopreservation involves preserving the sperms so they can be used later. This technique can prove to be a pathway for restoring the number of endangered species as their sperm can be preserved and used for fertilization later on. Objective: The propose of study is to find out the optimum thawing time, temperature and post thaw incubation period of cryopreserved buffalo sperm. Methods: Three buffalo bulls of the 1-2 year age were selected at Semen Production Unit, Qadirabad, Pakistan. Semen was collected by artificial vagina at 42 °C and 18 qualified ejaculates (two ejaculates/bull/week) for 3 weeks (replicate) were cryopreserved following a standard protocol. Frozen semen was divided in different groups, with each group being thawed at different temperature and time. Group I was subjected to slow thawing at 4-5°C, 2nd group to moderate thawing at 35ºC-37 ºC , 3rd group to rapid thawing while the 4th one was subjected to air thawing. Moreover, optimum incubation period of cryopreserved sperm was evaluated by thawing and incubating 42 straws in a water bath at 37ºC in with 2 straws being assessed every hour up till 10 hours. Results: Thawed semen samples were assessed based on different semen quality parameters. Acrosome integrity, plasma membrane integrity and progressive motility of cryopreserved buffalo sperm were significantly higher (P<0.05) in Group II which was subjected to moderate thawing. However, sperm quality parameters remained same (P > 0.05) with different incubation time (from 1 hr to 11 hr at 37°C). Conclusion: In conclusion, moderate thawing is effective in protecting the sperm cell from freeze thaw damages during cryopreservation. Sperm functional quality parameters were very acceptable after even 5 h of incubation indicated the usefulness of these incubated semen straws for field insemination in areas where availability of liquid nitrogen containers is problematic. Digital thermos maintained at 37°C could be alternative for short distance travelling for taking semen doses at insemination site. Further research efforts are needed to verify the fertilizing ability of this straw incubated (37°C) semen. | ||||
| Keywords | ||||
| Buffalo; Cryopreservation; Semen; Thawing Protocols | ||||
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