HYDROLYSIS OF SOME AGRICULTURAL CELLULOSIC WASTES BY Aspergillus awamor; CELLULASES PRODUCED BY SOLID-STATE FERMENTATION | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 2, Volume 27, Issue 8, August 2002, Page 5561-5574 PDF (1.44 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2002.256302 | ||||
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| Authors | ||||
| T. S. M. Shady,1; W. I. A. Saber,1; Nadia A. A. Ali1; Afaf-Hanem M. Ramdan2 | ||||
| 1Microbiol. Dept., Soil, Water and Environ. Res. Institute, Agric. Res. Center, Giza, Egypt. | ||||
| 2Home Economics, Fac. of Specific Education, Mansoura Univ., Mansoura, Egypt. | ||||
| Abstract | ||||
| The use of purified cellulose for bioconversion into cellulases increases the cost of enzyme production. Consequently, there have been attempts to develop a bioprocess to produce such enzymes using different lignocellulosic wastes. The results revealed that: High extracellular cellulases activities were observed after 4 days incubation. Com stalk (1.5%). sugar cane molasse (1 %), corn steep liquor (at 0.056% as nitrogen content) were found as the best inducers for these enzymes biosynthesis. pH 6.5 and 4.5 were found as the favourable pH for p-glucosidase and CMC-ase & FP-ase synthesis. respectively. 35°C. 40 and 35 & 50°C were found as the optimum temperature for these enzymes production, respectively. PH S.O and 5.5 , 50 and 60°C were found as the pH and temperature optima for p-glucosidase and CMC-ase & FP-ase activities, respectively. These enzymes were completely stable in the pH range between 5.0 - S.O, outside this pH range, all enzymes lost highest amount of their maximum activities. P-Glucosidase was completely stable up to 50·C. Some deleterious effects were happened to enzyme protein over this temperature degree. CMC-ase and FP-ase were highest stable up to 70·C, above, enzymes activities decreased sharply, This means that these enzymes were thermostable enzymes. Ca+2 and Mg+2 stimulated these enzymes activities. Others such as HG+2 and Fe +2 inhibited their activities with much more inhibition. These enzyme mixtures were successful to hydrolyzed untreated and treated cellulosic wastes. Alkali treated materials were hydrolyzed with higher extent ranged between 1.8 to 3.24 times than other untreated ones indicating that these enzymes play an important role for hydrolysis similar materials and recycling such materials to an important biotechnological substances. | ||||
| Keywords | ||||
| Aspergillus awamori; cellulases; solid-state fermentation; bioconversion; hydrolysis; alkali-treated; agricultural cellulosic wastes | ||||
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