THE INFLUENCE OF MELATONIN ON STORABILITY OF COOLED AND FROZEN BUFFALO SEMEN | ||||
| Journal of Animal and Poultry Production | ||||
| Article 4, Volume 27, Issue 10, October 2002, Page 6655-6663 PDF (2.5 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jappmu.2002.256762 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| I. S. EI-Shamaa, | ||||
| Animal Production Department, Faculty of Agriculture, Kafr EI-Sheikh, Tanta University | ||||
| Abstract | ||||
| Three semen ejaculates were collected weekly for eight successive weeks from three mature buffalo bulls to study the effect of melatonin on storability of buffalo bull semen. Only ejaculates with 70% of spermatozoa exhibiting progressive motility were included in the present study. The semen was extended by using Tris-egg yolk diluent to obtain 100 x 106 sperm J'er ml. Melatonin was added tc diluted semen as 0.0, 10, 20 and 30 J,lg/100 x 10 sperm. Thereafter, diluted semen (control and treatments) was split into tow portions. The first part was stored at refrigerator (4°C) temperature for five days and examined daily for motility, live sperm percentage, sperm abnormality % as well as GOT, GPT release and fructose content in seminal plasma. The second portion was frozen in 0.25 ml straws after 4 h equilibration period at 5°C post dilution. Straws were stored in liquid nitrogen for 24 h before thawing in water bath at 37°C for 30 sec. The results showed that the addition of 10 J,lg or 20 J,lg melatonin improved (P < 0.05) sperm motility, live sperm % and reduced percentage of sperm abnormality for 5 days of storage (at 4°C). The release of both GOT and GPT from spermatozoa into seminal plasma was significantly (P < 0.05) minimized in the presence of 20 J,lg melatonin compared with other treatments. Concerning fructose content in seminal plasma, addition of melatonin had a significant (P < 0.05) effect on conservation of energy in seminal plasma. The enzyme activities released to the seminal plasma were negatively correlated with sperm motility and positively correlated with sperm abnormality. The presence of 20 J,lg melatonin in the extender resulted in higher (P < 0.05) frozen-thawed motility compared to other treatments. It is concluded that buffalo semen could be successfully preserved for 5 days by addition of melatonin in Tris- extender at 4°C.or - 196°c | ||||
| Keywords | ||||
| Melatonin; buffalo semen; enzymes; frozen semen; storability | ||||
|
Statistics Article View: 36 PDF Download: 144 |
||||
