ISOLATION AND IDENTIFICATION OF LIPOLYTIC BACTERIA AND PRODUCTION OF LIPASE | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 1, Volume 27, Issue 9, September 2002, Page 6361-6380 PDF (3.33 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2002.257011 | ||||
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| Authors | ||||
| Aida H. Atify,; Fatma I. El-Hawary; I. I. Ismail; Amal Y. M. I. EI-Shafey | ||||
| Dept of Microbiology, Fac. of Agrlc., Mansoura Unlv., Mansoura, Egypt. | ||||
| Abstract | ||||
| Microbial lipases have highest potentiality due to their reacton selectivity and mild conditions. Accordingly, Ihis worX was suggested 10 isolate and identity some microorganisms capable of lipase production and to optimize the culture conditions for these microorganisms to produce lipase. The organisms were Isolated from clayey 5011, and identified as Pseudomona~ aeruginosa, P. fluorescens and Bacillus cereus. tncubauon periods for lipase production from, P. fluorescens P. aeruginosa and B. cereus were one day, 2 days and 3 days, respectively. The maximal yield of lipase production from P. ae(!1ginosa, P. fluorescens and B. cereus was observed at 30°C. The optimal pH value was 6.0. The maximum enzyme yield was obtained when the production medium was supplied with 1 % glucose as a carbon source in the presence of olive oil. The highest growth and lipase production were observed With ol/ve oil as inducer ali % concentration. Three organic and inorganic nitrogen sources were added separalely to the fermentation media, peptone plus urea gave maximum production of lipase (10.9 urno: FFA I mi I min) at nitrogen eN) concentrallon of 0.92% ror P. 8e(!1ginosa, while P. fluorescFJns recorded 8.50 )J.mol FFN mIl min and B. C&r&us 4.98 urnol FFA J mil min. | ||||
| Keywords | ||||
| Lipa; e procucuon; lipolytiC bacteria; P. aerugino.saP. fluorescens and 8. cereus | ||||
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