PURIFICATION AND IMMOBILIZATION OF LIPASE PRODUCED FROM Pseudomonas aeruginosa | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 2, Volume 27, Issue 9, September 2002, Page 6381-6392 PDF (876.32 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2002.257015 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Fatma I. El-Hawary,; Aida H. Afify; I. I. Ismail; Amal Y. M. I. EI-Shafey | ||||
| Dept. of Microbiology, Fac. of Agric., Mansoura Univ., Mansoura, Egypt | ||||
| Abstract | ||||
| The lipases of P. aeruginosa were isolated, immobilized and characterized. They were applied to hydrolysis of crude oils i.e. cotton oil, maize oil, palm oil, sunflower oil and olive oil. The lipase produced by P. aeruginos8 was precipitated from the growth media using by cold acetone and was immobilized on celite. The characteristics of free and immobilized lipases were studied as follows: The optimum ternperature for Iipases activity was 37°C for free lipase. whereas it was 44°C for immobilized one. The optimum pH value for free and Immobilized lipases was 7.4. The stability to pH was higher in Ihe Immobifized enzyme than the free one. Immobilized lipase was stable between pH 6.8-7.8 and tree lipase between 7.2-7.6. Both free and immobilized lipases were stable at 40·C but immobilized one was more stable at (4'J-SO°C). The optimum substrate concentrations for free and immobilized Iipases were 0.25% and 0.30"/0, respectively. Free and immobilized lipases were able 10 hydrolyse all natural oils tested. Olive olf was the most hydrolyzed one than other oils. | ||||
| Keywords | ||||
| Immobilized lipase; oils. Pseudomonas aeruginosa | ||||
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