ETHANOL PRODUCTION FROM CELLULOSE VIA INTERGENERIC PROTOPLAST FUSION BETWEEN Trichoderma reese; AND Saccharomyces cerevisiae FUSANTS | ||||
| Journal of Plant Production | ||||
| Article 9, Volume 27, Issue 11, November 2002, Page 7335-7345 PDF (11.96 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2002.257032 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Fatma N. Talkhan,; A. M. EI-Bondkly; A. A. Ismail | ||||
| Genetics and Cytology Department, National Research Center, Dokki, Cairo, Egypt. | ||||
| Abstract | ||||
| The present study aimed to investigate the possibility of inducing new genetic recombinants having the ability to ferment cellulose into ethanol upon fusion of T. reese; and S. cerevisiae protoplasts. Twelve stable fusants were induced and lested for ethanol as well as cellulase enzymes productivity. Out of these fusants 8 isolates produced ethanol with amounts ranged from 45% (F/4 and F/5) to 90% (Ff7) of the parent S. cerevisiee, whereas the remaing 4 did not produce ethanol. Meantime Ihese none ethanol producing fusants showed cellulase activities of the three enzymes (FPase, CMCase and ~-9Iucosidase). On th other hand, 3 fusants (F/1, F/6 and Fn) out of the eight ethanol producer showed cellulase activities of these three enzymes, whereas the other five fusants (F/2, F/4, F/5, F/9 and F/10) showed cellulase activities of the two enzymes FPar.e and CMCase only. None of these 12 fusants overyieled the parental strain T. reese; (3126) in cellulases production. | ||||
| Keywords | ||||
| Trichoderma reesei; Saccharomyces cerevisiae Cellulase enzymes; Ethanol; Intergeneric cross; Protoplast fusion | ||||
|
Statistics Article View: 31 PDF Download: 128 |
||||
