PHYTOCHEMICAL STUDIES ON Verbascum sinaticum BENTH | ||||
| Journal of Plant Production | ||||
| Article 14, Volume 27, Issue 11, November 2002, Page 7403-7415 PDF (13.11 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2002.257043 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Nahed A. E. Salem, | ||||
| Desert Research Center | ||||
| Abstract | ||||
| The preliminary phytochemical screening of Verbascum sinaticum which collected from Catherin and Wadi Fieran showed that it contained sterols, flavonoids, chlorides, tannins, resine, saponines, alkaloids, and carbohydrates and/or glycosides. The paper chromatography of free sugars showed that Verbascum sinaticum contained glucose, fructose, rhamnose and arabinose in leave at the two studied habitats, while it was obvious that rhamnose was absent in stem at Catherin and rhamnose and fructose were absent In stem at Wadi Fleran.Conceming the combined sugars glucose, rhamnose, sucrose and arabinose were detected in the leaves, while the stem contained the same components except rhamnose at Catherin habitat. On the other hand at Wadi Fieran habitat the results revealed the presence of glucose, fructose, rhamnose, sucrose and arabinose in leave, while the stem contained the same components except fructose and rhamnose. Investigation of free amino acid showed the present of proline, alanine and lysine in leave and stem at the two studied habitats, while aspartic acid, serine, glycine, valine, isoleucine and leucine present only in the stem at the two habitats but varied in leave at the two habitats. Concerning the protein-amino acids, Verbascum sinaticum contained seventeen amino acid with different concentrations. Proline was the highest one of the bound amino acids 25.1 and 18.3% in leave at Catherin and Wadi Fieran sample respectively. Meanwhile,leucine was the highest one 18.6 and 15.1% in the stem at the same habitats, respectively. The values of the fundamental chemical properties of the extracted lipid of Vemascum sinsticum were lower in leaves than that found in stems, while acid, iodine, ester and saponification values were higher at Wadi Fieran habitat than that sample at Catherin habitat. The unsaponifiables content of Verbascum sinaticum was determined and the relative percentages of each component were calculated. There was no significant . difference between the two habitats except the presence of tetracosanoic at Catherin only and the presence of cholesterol in leave and stem at catherin and Wadi Fieran respectively. The Gas-liqued chromatographical analysis of Verbascum sinaticum lipids revealed the presence of long chain fatty acid, where stearic acid was the major fatty acid in leaves at two habitats, while linoleic acid was the major component in stem at two habitats. Concerning the metabolic products studies, it was found that the percentage of total nitrogen, total protein, total lipid and total, soluble and insoiuble carbohydrates were higher in the leaves than those in the stems at both studied habitats, while they were higher at Catherin than those at Wadi Fieran habitats. Concerning the active constituents it was found that the percentages of total flavonoids, total alkaloids, total saponins and total tannins were higher at the leaves than those in the stem, while they were higher at Calherin than those at Wadi Fieran habitats. | ||||
| Keywords | ||||
| Phytochemical; Verbascum; aminoacid; fatty acid; carbohydrate | ||||
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