USING SOME ISOLATES AND TRANSFORMANTS OF AZOTOBACTER TO REDUCE CHEMICAL NITROGEN FERTILIZER RATES IN GARLIC PRODUCTION

Foly,, H. M.H.; Dakhli, O. F.; Awad, EL. M.; Abdel-Mageed, Y. T.; Hassan, E. A.

doi:10.21608/jpp.2002.257088

	USING SOME ISOLATES AND TRANSFORMANTS OF AZOTOBACTER TO REDUCE CHEMICAL NITROGEN FERTILIZER RATES IN GARLIC PRODUCTION
Journal of Plant Production
Article 25, Volume 27, Issue 11, November 2002, Page 7667-7684 PDF (20.68 MB)
Document Type: Original Article
DOI: 10.21608/jpp.2002.257088
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Authors
H. M.H. Foly,¹; O. F. Dakhli²; EL. M. Awad¹; Y. T. Abdel-Mageed³; E. A. Hassan⁴
¹HortJ. Res. Institute., Giza
²Dept. of Genetic; Fac. of Agrlc., Mlnla Univ
³Hortlcultural Depart. (Vegetables), Fac. of Agrlc., Mlnia Univ., Minia, Egypt,
⁴HortJ. Res. Institute., Giza,
Abstract
The present investigation was conducted at the laboratories of Genetics and Horticulture departments. Fac. of Agric .• Minia University and farm of Mallawy Horticulture Research Station. This study was carried out during the two successive winter seasons of 200012001 and 200112002 to study the effect of application dl1ferent genotypes of Azotobacter(Transformatlon procedure) and dl1ferent rates of chemical N fertilizers in-gartic production (cv. Chinese). Wild type. four transtormants of two Azotooocterspecies (two from each species) and three nitrogen rates i.e. 25%. 50% and 100% of the recommended dose (120 kg NlFed.) were used. The effects of Azotobacter transformants and chemical nitrogen fertilizers and its interactions on growth characters fresh and cured yield. yield components and nitrate and nitrite content In cloves were studied. The obtained results demonstrated that: (1)· DNA extract from two Azotobacter species (A. vinelandii and A. chroococcum) grown under 200 mg/ml chloramphenical was used to transform resistant to antibiotics isolates of the same species sensitive to antibiotics. The highest frequency of transform ants (1 00X10~ was obtained when A. chroococcum was a donor and A. cnroococeum was a recipient. Therefore, the highest frequencies were obtained from the intraspecific transformation and the lowest frequencies 'oIIere obtained from the interspecific transformation. (2)- Inoculation garlic plants cv "Chinese" with either transformants or . wild type strain of Azotobacter improved most growth characters and yield addition to reducing nitrate content in cloves. (3)- Fertilization with 60 and 120 kg. NlFed. gave significantly higher values compared with 30 kg. NlFed. in respect of bulb weight and clove weight. as well as total fresh and curd yields. (4)- The differences between 60 and 120 kg. NlFed. were insignifi~nt in most growth and yield measurements. (5) The Azotobacter transformants differed significantly each from other in their effects . on garlic growth and yields. The transformants (T3) and (T4) showed the highest values in both seasons. (6) The nitrate contents in garlic cloves samples were increased with increasing the applied N-chemical fertilizer' to the combined biofertilizer treatment. An Increase (21.8%) In nitrate level was detected In garliC samples produced from plants treated with chemical fertilizers compared with the Azotobacter wild type treatment. Generally the plants fertilized with chemical fertilizer contained highest value' of nitrate (217 mglkg) than those biofertilized with Azotobacter. In conclusion, inoculation garlic plants with Azotobacter bacteria particularly transformants could be reduced using the chemical nitrogen fertilizer by about 25% from the recommended dose to obtain the same values of growth and yield. Moreover. nitrate content in cloves was reduced after inoculation garlic plants with Azotobacter transformants.


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