DIAGNOSIS OF BOVINE BRUCELLOSIS USING MILK RING AND SEROLOGICAL TESTS WITH SPECIAL REFERENCE TO COLOSTRUM AND MILK IMMUNOGLOBULINS. | ||||
| Journal of Food and Dairy Sciences | ||||
| Article 3, Volume 27, Issue 11, November 2002, Page 7529-7536 PDF (8.97 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jfds.2002.257129 | ||||
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| Authors | ||||
| M. M. EI-Loly,1; Y. A. Ghazi2 | ||||
| 1Dairy Dept. National Research Centre, Dokkl, Cairo, Egypt. | ||||
| 2Dept. Animal Reproduction & A.I. National Research Centre, Dokkl, Cairo, Egypt. | ||||
| Abstract | ||||
| Blood and whey serological tests (RBPT, TAT, Riv.n as well as milk ring test were preformed on individual samples obtained from 84 dairy cows for diagnosis of bovine brucellosis. Also, samples were taken from colostrum and milk to evaluate the effect of brucellosis on immunoglobulin concentrations using ELISA. In addition, milk samples were used for isolation and identification of brucella organisms. Results revealed that the incidence of brucellosis using blood serum were 21.42, 25.0 and 20.23% with RBPT, TAT and Riv.T, respectively. Meanwhile, in whey the corresponding figures were 11.90, 10.71 and 13.09%. The milk ring test revealed that the incidence of brucellosis was 13.09 %. Brucellosis caused significant increases in all immunoglobulin fractions in mammary secretions, especially IgA in colostrum as well as IgG and IgA and total Igs in milk of infected cows, as compared to that of the normal control cows. While, IgM was obviously lower in milk of brucella positive cows. Only two isolates of brucella melitensis biovar 3 were isolated from 21 positive TAT reactors milk samples. It was concluded that for proper rapid screening of brucellosis, MRT should not be performed alone. | ||||
| Keywords | ||||
| Cow; colostrum; milk; blood; brucellosis; serological tests; ELISA; immunoglobulins | ||||
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