Evaluation of the immunological status of the common carp (Cyprinus carpio) infested with Lernaea cyprinacea | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 75, Volume 26, Issue 4, July and August 2022, Page 1305-1318 PDF (1.69 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejabf.2022.257145 | ||||
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| Author | ||||
| Marwa M. Attia et al. | ||||
| Abstract | ||||
| This study was initiated to assess the major histocompatibility class II (MHC-II) and Interleukin-1β (IL-1β) for the evaluation of the immunological reaction to histopathological reaction of common carp infested by Lernea cyprinacea (L. cyprinacea). Thus, a total of 100 common carp (Cyprinus carpio) fish were collected from Cairo and Giza Governorates in Egypt and investigated for the occurrence of different ectoparasites. Naturally, the infested C. carpio showed hemorrhagic skin with ulcer formation around the copepod; sloughing of the scales. The presence of any external parasites was detected on all fish body. Skin; muscle and gills samples of Cyprinus carpio (C.carpio) were collected for histopathological investigation. The parasitic female L. cyperinacea is elongated with its total length ranging from 10–18 mm (14 ± 1.5 mm). In the anterior end; it forms an anchor in which it pierces the skin to insert into the muscle; it has four horns; two dorsal and two ventral. The skin of the infested L. cyprinacea was pale in color; muscles contained multiple millet seed-sized parasitic cysts embedded in C. carpio muscles. Histopathological examination of the skin and muscles of C. carpio revealed parasitic cysts of L. cyprinacea which were attached between the intense degenerated fins with hyperplasia, hypertrophy and loss of its normal lamellar structure. Skin; gills and mucous were dissected from infested fish with L. cyprinacea, all samples were stored aseptically at -20 °C for subsequent investigation. The samples were classified into 4 groups: group 1: samples with 1-5 Lernaea; group 2: samples with > 5-10 Lernaea; group 3: > 10 copepods and group 4: control healthy non-infested fish. The examined genes were elevated according to the degree of infestation levels. In conclusion, infestation with L. cyprinacea in C. carpiois was accompanied by an up-regulation of gene expression of MHC-II and IL-1β in C. carpio infested gills, skin and mucous. | ||||
| Keywords | ||||
| Lernaea cyprinacea; Common carp; Gene expression; Immunological status; MHC-II; IL-1β | ||||
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