EVALUATION OF LACTOBACILLUS ACIDOPHILUS, CHITOSAN AND/OR AZITHROMYCIN IN TREATING CRYPTOSPORIDIOSIS IN NEONATAL MICE | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Volume 52, Issue 2, August 2022, Page 183-192 PDF (1.12 MB) | ||||
| DOI: 10.21608/jesp.2022.257331 | ||||
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| Authors | ||||
| NAGLAA F. A. IMAM1; RABAB S . ZALAT2; MOHAMED SHERIF NEGM3; NARDEEN ZAKKA BOCKTOR1 | ||||
| 1Department of Medical Parasitology, Faculty of Medicine | ||||
| 2Department of Parasitology, Theodor Bilharz Research Institute, Giza, Egypt | ||||
| 3Department of Pathology, Faculty of Medicine3, Cairo University | ||||
| Abstract | ||||
| There are various limitations on the efficacy of anticryptosporidal drugs with a critical need to have better alternatives. This study evaluated the effect of Lactobacillus acidophilus (L. acidophilus), a probiotic and chitosan, a natural polysaccharide as compared to azithromycin (AZT), as single therapy and in combination in treatment of cryptosporidiosis on infected neonatal mice. The three therapeutics individually showed significant decrease in Cryptosporidium oocysts shedding compared to control (P <0.001). However, L. acidophilus caused a higher reduction (91.07%) compared to both AZT (83.19%) and chitosan (65.61%). Also, they reduced the oocysts numbers detected in the intestine, resolved totally the neutrophilic activity, without affecting the intestinal inflammation, but caused liver inflammation improvement. AZT/L. acidophilus combination caused highest reduction in oocysts shedding (93.44%), followed by chitosan/L. acidophilus combination (92.76%). These results represented a significant improvement compared to AZT single therapy (P-values 0.012 & 0.024 respectively) or chitosan single therapy (P <0.001 for both combinations). Besides, all therapeutic combinations removed any apparent parasite in the intestine and halted acute neutrophilic activity. Intestinal inflammation totally disappeared with chitosan/ L. acidophilus combination. Liver inflammations improved with drug combinations, but did not resolve totally as mostly observed with single therapy | ||||
| Keywords | ||||
| Cryptosporidium; Neonatal mice; Lactobacillus acidophilus; Chitosan; Azithromycin | ||||
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