CLOVE OIL OR COMBINED WITH PRAZIQUANTEL DOWN REGULATED TGF-Β1 AND REVERSED FIBROGENIC DYNAMIC PROGRESSION CAUSED BY CHRONIC SCHISTOSOMIASIS MANSONI IN MICE | ||||
| Journal of the Egyptian Society of Parasitology | ||||
| Volume 52, Issue 2, August 2022, Page 231-238 PDF (1.33 MB) | ||||
| DOI: 10.21608/jesp.2022.257335 | ||||
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| Authors | ||||
| REHAM REFAAT MOSTAFA1; MAHA HASSAN ELAROUSY1; MAISA AHMED SHALABY2; AMANY AHMED ABDELAAL3; SOHEIR S. MAHMOUD2; MANAL ABDELMAGED BADAWI4; SHERIF MOHAMED ISMAIL5; REHAM K. NAHNOUSH3 | ||||
| 1Department of Medical Parasitology, Faculty of Medicine, Cairo University | ||||
| 2Department of Parasitology, Theodor Bilharz Research Institute (TBRI) | ||||
| 3Department of Medical Parasitology, Faculty of Medicine | ||||
| 4Department of Pathology , National Research Institute | ||||
| 5Department of Pathology, Faculty of Medicine, Cairo University | ||||
| Abstract | ||||
| The most serious form of chronic schistosomiasis is the life-threatening hepatosplenic disease, accompanied by severe periportal fibrosis, a permanent condition once established. Reversion of such wound healing process “fibrolysis” is possible if a balance between hepatic cellular regeneration and the process of scar formation is ensued. In the current work, clove oil alone and combined with PZQ were used to investigate their anti-fibrotic effect on hepatic fibrosis resulted from acute and chronic schistosomiasis mansoni in mice. To determine the state of hepatic fibroblasts, local expression of TGF-β1, a chief profibrogenic molecule was quantified using digital realtime image analysis and compared with the conventional parasitological and histopathological analysis. PZQ monotherapy caused a significant reduction in ova count, granuloma number and size. Local expression of TGF-β1 gave better data as compared to acute and chronic infected treated mice (11.91±2.53- 20.34±3.05 vs. 7.51±2.11-11.23±2.23) with clove alone treatment & 4.95±1.95-7.51±1.92 in combined therapy (p=0.0000)), indicated low potential in achieving liver tissue repair. Significant drop in TGF-β1 expression with clove oil treatment, especially when combined with PZQ, indicated anti-fibrotic potentiality and good impact on liver cells proceeded towards regeneration supported by such drop. A significant positive correlation between mean TGF-β1 values and mean granuloma parameters in number and size of different infected groups (R² = 0.680 & 0.988, respectively in acute infection, 0.363 & 0.505 respectively, in chronic phase of infection, P value <0.05). | ||||
| Keywords | ||||
| Mice; Schistosoma mansoni; Clove oil; Praziquantel; Hepatic fibrosis; TGF-β1 | ||||
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