VALUE OF MICROSCOPY AND COPROANTIGEN FOR DETECTION OF CRYPTOSPORIDIUM AND GIARDIA IN STOOL SAMPLES | ||||
Journal of the Egyptian Society of Parasitology | ||||
Volume 52, Issue 2, August 2022, Page 261-266 PDF (3.42 MB) | ||||
DOI: 10.21608/jesp.2022.257454 | ||||
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Authors | ||||
ENGY AHMED ABDALLAH NAHAS1; SAYEDA M. AUFY2; MOUSA A. M. ISMAIL2; REHAM KAMAL MOHAMED2; DINA M. H. EL AKKAD2 | ||||
1Department of Parasitology, Faculty of Medicine, 6th October University | ||||
2Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt | ||||
Abstract | ||||
Giardiasis and cryptosporidiosis are common protozoa causing gastrointestinal troubles with symptoms ranged from asymptomatic to long-lasting diarrhea with mal-absorption. The present work was carried on a total of 96 cases; 62 of them presented with GIT symptoms with or without diarrhea, 50% of them was previously diagnosed as giardiasis or cryptosporidiosis infected and treated 1-2 months ago, 11 cases were free from GIT symptoms and 23 cases suffered from chronic debilitating disease as cancer or renal disease, some of them have previous history of gastro-intestinal disturbances. The rapid immunochromatographic test (ICT) and copro-ELISA detected G. lamblia and C. parvum antigens in all fecal samples compared to formol ether concentration and Modified Ziehl-Neelsen (MZN) staining technique. The sensitivity of G. lamblia in stool samples was 60%, 98.3% & 96.6% using concentration technique, ICT & ELISA respectively, and specificity was 98.75%, 96.43% & 96.43% respectively. The sensitivity of C. parvum with MZN stain and antigen detection by ICT& ELISA was 46.2%, 96.4% & 100% respectively. Specificity of the tests in detecting C. parvum was better than Giardia; 96.4% -100% respectively. All tests have 100% PPV and 80-888% NPV for the immunological techniques with superiority of ICT over ELISA. | ||||
Keywords | ||||
Giardia; Cryptosporidium; ELISA; Coproantigen; ICT; MZN; formol ether concentration | ||||
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