II- IN VITRO PROPAGATION OF DATE PALM (Phoenix dactylifera L.) cv. “ZAGHLOUL” BY SOMATIC EMBRYOGENESIS | ||||
| Journal of Plant Production | ||||
| Article 29, Volume 27, Issue 12, December 2002, Page 8621-8631 PDF (671.14 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2002.257634 | ||||
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| Author | ||||
| M. B. El- Sabrout, | ||||
| Pomology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt. | ||||
| Abstract | ||||
| The present investigation was carried out during three successive years (1999- 2001), in order to study the effect of the cytokinin (BA) to auxin (NAA) ratio in culture medium (Murashige and Skoog, 1962) on somatic embryogenesis in date palm cv. “Zaghloul” cultures. The main results can be summarized in the following points: 1- Shoot tip explant proved to be the best in terms of callus formation percentage as compared with the leaf primordia explant. 2- MS (1962) medium supplemented with 3.0 mg1-1 2 ip + 30.0 mg1-1 NAA, the callus formation percentage was significantly the highest (100% in shoot tip explant and 50.0% in leaf primordia explant). 3- Embryogenesis percentage was significantly the highest (87.5%) on MS (1962) medium supplemented with 3.0 mg1-1 B A + 1.0 mg1-1 NAA. On the same medium, average number of germinated embyoids per callus was significantly the highest (2.75). 4- MS medium supplemented with 3.0 mg1-1 NAA in medium produced the highest rooting percentage (100 %), greatest average roots number per shoot (12.0) and the longest roots (10.0 cm). 5- Finally, 70% of the obtained plants of date palm cv. “Zaghloul” were successfully transplanted to soil. | ||||
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