GENETIC CONSTRUCTION OF BROAD HOST RANGE Bradyrhizobium japonicum | ||||
| Journal of Plant Production | ||||
| Article 9, Volume 26, Issue 9, September 2001, Page 5437-5447 PDF (710.38 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2001.257775 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| M. S. Abd El-Salam,1; S. A. Ibrahim2; M. M. Abd El-Halim1; S. Kh. Abd-El-Aal1; A. G. Attallah1 | ||||
| 1Microbial Genetics Dept., National Research Center , Cairo , Egypt | ||||
| 2Genetics Dept., Faculty of Agriculture, Ain Shams Univ., Cairo, Egypt | ||||
| Abstract | ||||
| Protoplast fusion and conjugation were used to expand the Rhizobium host range. B.japonicum AS25 and R.leguminosarum biovar vicia ICARDA441 were fused and six, genetically stable, fusants were isolated. No fusants were obtained when B.japonicum BR20 and Agrobacterium tumefaciens CB were fused. Conjugation technique was done between Agrobacterium tumefaciens CB and twenty-five B.japonicum strains, which isolated from different Egyptian governorates. Fourteen conjugants were selected according to their variant antibiotic resistance (Genetic marker). The six fusants were able to nodulate both soybean and Vicia faba, while the fourteen conjugants were able to nodulate both clover and soybean. Plasmid profiles and phyllogenetic analysis of the parents; B.japonicum AS25 and R.leguminosarum biovar vicia ICARDA441, and their six fusants were done. All fusants and conjugants were characterized and their relatedness were determined according to plasmid profiles. | ||||
| Keywords | ||||
| Genetic construction; host range; Bradyrhizobium japonicum; protoplast fusion; conjugation | ||||
|
Statistics Article View: 41 PDF Download: 129 |
||||
