Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.
Badawy, A., Zakaria, A., EI-Belely, E., El-Gamal, A., Hammad, A. (2023). Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.. EKB Journal Management System, 66(6), 217-223. doi: 10.21608/ejchem.2022.155468.6711
Ahmad Muhammad Badawy; Abeer Emam Zakaria; Ehab Fathy EI-Belely; Ahmed Darwish El-Gamal; Ali Ahmed Hammad. "Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.". EKB Journal Management System, 66, 6, 2023, 217-223. doi: 10.21608/ejchem.2022.155468.6711
Badawy, A., Zakaria, A., EI-Belely, E., El-Gamal, A., Hammad, A. (2023). 'Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.', EKB Journal Management System, 66(6), pp. 217-223. doi: 10.21608/ejchem.2022.155468.6711
Badawy, A., Zakaria, A., EI-Belely, E., El-Gamal, A., Hammad, A. Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.. EKB Journal Management System, 2023; 66(6): 217-223. doi: 10.21608/ejchem.2022.155468.6711

Statistical Optimization of Total Fatty Acids and Protein Content in Spirulina platensis Biomass.

Egyptian Journal of Chemistry
Article 23, Volume 66, Issue 6, June 2023, Page 217-223  XML PDF (768.25 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.155468.6711
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Authors
Ahmad Muhammad Badawy email 1; Abeer Emam Zakaria2; Ehab Fathy EI-Belely3; Ahmed Darwish El-Gamal3; Ali Ahmed Hammad4
1Radiation Microbiology, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority , Cairo, Egypt
2Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority.
3Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt.
4Radiation Microbiology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority
Abstract
Protein and fatty acid deficiency in human nutrition is a major concern for developing countries. The filamentous blue-green microalga Spirulina platensis is one of the best algal species that can be used as a food supplement. This study aims to optimize biomass, the total protein, and the fatty acid content of Spirulina platensis. Statistics optimization was carried out using the response surface methodology via dry weight and the Kjeldahl method for protein determination, while the fatty acid composition was determined by the conversion of oil to fatty acid methyl esters (FAMEs), which was measured on a gas chromatography (GC) device. The results revealed that the optimum conditions for maximum biomass, total protein, and fatty acid content were in the presence of NaHCO3 at (16.8 g/l) and NaNO3 (2.5 g/l) at temperature (20°C), pH (9), and LI (6000 lux). A prospective study to estimate both essential amino acids and essential fatty acids in S. platensis biomass is recommended.
Keywords
Biomass; Fatty acids; Proteins; Response Surface Methodology; Spirulina platensis
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