UTILIZATION OF COTTON STALKS | ||||
| Journal of Soil Sciences and Agricultural Engineering | ||||
| Article 10, Volume 25, Issue 7, July 2000, Page 4777-4783 PDF (375.16 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jssae.2000.259456 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Sekina A. Mohamed | ||||
| Soils, Water and Environ. Res. Inst., Agric. Res. Center, Giza, Egypt. | ||||
| Abstract | ||||
| Three different methods has been used for the hydrolysis of the cotton stalks to reducing sugar and valuable products. These methods involves: (1) Biological degradation by cultivation of Aspergillus niger on cotton stalks in simple media. The bio-products of A. niger growth in cultures filtrate were evaluated after 6 days of incubation with shaking (~120 rmp) at 300C. (2) Dual-step acid hydrolysis (H2SO4 72%, 4%, v/v) at different time and temperature of hydrolysis.(3)- Enzymatic hydrolysis (two sources of cellulases, separately) for 3 days at 370C and pH 4.6. In biological degradation, the highest bioproducts in culture filtrate (1.72 mg/ml reducing sugar; 5.01 and 1.27 U/ml of µ - amylase and amyloglucosidase; 5.92 and 9.31 mg sugar / ml / h of Cx and X enzymes and 2.78 and 2.37 mg sugar / ml / 24h of C1 and Av enzymes, respectively) were obtained in medium was contained: 10 g cotton stalks, 10g CaCO3 and 100 ml water. In dual step acid hydrolysis, the high yield of reducing sugar (80.0 %) was obtained with 85 min - first step acid hydrolysis and 60 min second step acid hydrolysis. While, high yield of reducing sugar in enzymatic hydrolysis (14.97 mg / ml) was occurred with solid cellulase. | ||||
| Keywords | ||||
| cotton stalks; Aspergillus niger; acid hydrolysis; enzymatic hydrolysis; low-cost media | ||||
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