ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING RHIZOBACTERIA PRODUCING INDOLE-3-ACETIC ACID FROM PLANTS GROWING IN EGYPT
El Khawas,, H., Ibrahim, I., Anwar, H., Hegazi, N. (2000). ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING RHIZOBACTERIA PRODUCING INDOLE-3-ACETIC ACID FROM PLANTS GROWING IN EGYPT. EKB Journal Management System, 25(10), 6493-6502. doi: 10.21608/jacb.2000.259828
H. M. El Khawas,; I. A. Ibrahim; Hala M. Anwar; N. A. Hegazi. "ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING RHIZOBACTERIA PRODUCING INDOLE-3-ACETIC ACID FROM PLANTS GROWING IN EGYPT". EKB Journal Management System, 25, 10, 2000, 6493-6502. doi: 10.21608/jacb.2000.259828
El Khawas,, H., Ibrahim, I., Anwar, H., Hegazi, N. (2000). 'ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING RHIZOBACTERIA PRODUCING INDOLE-3-ACETIC ACID FROM PLANTS GROWING IN EGYPT', EKB Journal Management System, 25(10), pp. 6493-6502. doi: 10.21608/jacb.2000.259828
El Khawas,, H., Ibrahim, I., Anwar, H., Hegazi, N. ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING RHIZOBACTERIA PRODUCING INDOLE-3-ACETIC ACID FROM PLANTS GROWING IN EGYPT. EKB Journal Management System, 2000; 25(10): 6493-6502. doi: 10.21608/jacb.2000.259828

ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING RHIZOBACTERIA PRODUCING INDOLE-3-ACETIC ACID FROM PLANTS GROWING IN EGYPT

Journal of Agricultural Chemistry and Biotechnology
Article 6, Volume 25, Issue 10, October 2000, Page 6493-6502  XML PDF (418.31 K)
Document Type: Original Article
DOI: 10.21608/jacb.2000.259828
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Authors
H. M. El Khawas,1; I. A. Ibrahim2; Hala M. Anwar2; N. A. Hegazi3
1Department of Microbiology, Faculty of Agriculture, Cairo University, Giza, Egypt.
2Tissue Culture Laboratory, Agriculture Development Systems Project, Ministry of Agriculture, Cairo, Egypt
3Department of Microbiology, Faculty of Agriculture, Cairo University, Giza, Egypt
Abstract
The present work aims to surveying plant growth-promoting rhizobacteria (PGPR) for their ability to produce the plant growth regulator indole-3-acetic acid (IAA). One hundred and forty eight isolates from such bacteria were obtained representing the rhizosphere and rhizoplane of maize, wheat, barley, sorghum and sugar cane form different locations in Egypt (Giza, Faioum, Kafer El-Sheikh and Ismailia). Twenty isolates were selected according to their ability to produce high amount of microbial IAA. The determination of IAA was carried out by calorimetric method and chromatographic analysis (GLC). These isolates were grown on minimal medium with different carbon sources. Glucose was the best carbon source for growing the most isolates and the presence of tryptophan was necessary for production of IAA. Seven isolates which produce IAA more than others were purified and identified applying both conventional morphological, cultural, biochemical methods and API microtube systems. Accordingly, they were placed under the species of Klebsieilla pneumomiae, Azospirillum spp. Enterobacter cloacae, and Pseudomonas fluorescens.
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